Example sentences for: mutagenesis

How can you use “mutagenesis” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • However, to maximise the utility of tagged sequence mutagenesis, one would like to predict, from the sequence alone, the functional consequences of the inserted targeting vector on cellular gene expression.

  • Mutagenesis

  • Although Cdc5 activity (1) is necessary to free nucleolar Cdc14 in late mitosis, (2) can elicit ectopic release of Cdc14 in pre-anaphase cells when overproduced, (3) regulates the phosphorylation state of Net1 during anaphase, and (4) can disassemble native and recombinant RENT complexes in vitro by phosphorylating Net1, a combination of in vivo and in vitro phosphosite mapping and mutagenesis experiments suggest that Cdc5 mediates exit from mitosis primarily by phosphorylating targets other than Net1.

  • The bacteria used in this study were the opacity factor positive M49 Streptococcus pyogenes isolate CS101 and an isogenic β-hemolytic negative variant generated by transposon mutagenesis, CS101 sagA/pel ::Tn 917 [ 4 ] . Todd-Hewitt broth containing 0.3% yeast extract (THY) was obtained from DIFCO (Detroit MI).

  • Initially, alanine-scanning mutagenesis was used for this study as alanine substitutions are generally thought to minimally effect protein folding [ 27 ] . Sequence conservation from the alignment of LcrG with the


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