Words similar to cytoskeleton
Example sentences for: cytoskeleton
How can you use “cytoskeleton” in a sentence? Here are some example sentences to help you improve your vocabulary:
The assembly of myosin II monomers into filaments is regulated by phosphorylation of its heavy chains at three threonine residues at the C-terminus of the tail [ 9 10 ] . Dephosphorylation of these threonines is a prerequisite of filament assembly, as confirmed by the phenotypes of a 3xAsp mutant, in which the three threonines are replaced by three aspartate residues (mimicking the phosphorylated state) [ 11 ] . In vitro the 3xAsp myosin II is severely impaired for filament assembly, and in vivo 3xAsp myosin II fails to assemble or localize to the cortical cytoskeleton.
Western blotting of the biochemical fractions prior to RNA isolation confirmed that latrunculin B treatment resulted in a dramatic decrease in the amount of β-actin protein that was extracted in the cytoskeleton fraction.
There is also evidence that the actin cytoskeleton is involved in mitosis and during separation of daughter cells after the successful segregation of chromosomes into daughter nuclei [ 3].
Though BDM does not inhibit nm-myosinII, in permeabilized yeast ( S. cerviseae ) BDM has been shown to decrease incorporation of exogenous actin into the cytoskeleton [ 20 ] . This process of actin incorporation requires proteins involved in actin filament formation and proteins that may localize a nucleation complex [ 20 21 ] . To determine if BDM has a similar effect on actin incorporation in mammalian cells, and if so an effect on actin nucleation, spreading Swiss3T3 cells were treated briefly with a media control or 15 mM BDM and then permeabilized with the detergent saponin in the presence of fluorescently labeled actin.
Indeed, TGFβ 1 antibodies, cytochalasin D and BAPTA-AM [ 66 ] abolished the association on the cytoskeleton connected with integrin α V β 6 and activation of p125 FAK.
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