Example sentences for: cytosolic

How can you use “cytosolic” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Equal volumes of each sample (cytosolic and cytoskeleton-associated components) were subjected to SDS-PAGE, transferred to a nitrocellulose membrane (Bio-Rad) and immunoblotted with primary antibody to β-actin and detected using horseradish peroxidase-conjugated secondary antibody (Vector Laboratories) and SuperSignal West Dura (Pierce) as a chemiluminescent substrate.

  • Thiamine entry into mammalian cells occurs by a saturable, high affinity transporter that is deficient in humans with thiamine-responsive megaloblastic anemia (TRMA) [ 7 8 9 10 11 12 ] . Thiamine uptake by mitochondria has been demonstrated [ 13 ] , yet thiamine diphosphokinase is cytosolic and mitochondria cannot convert thiamine to ThDP [ 14 15 16 ] . Barile and coworkers demonstrated saturable uptake of ThDP by rat liver mitochondria characterized by a K m of around 20 μM.

  • Stimulation of P2X purinergic receptors by ATP causes similar changes in cytosolic Ca 2+and vital dye uptake [ 10, 11, 12, 13, 14, 15, 16, 17] suggesting that MTX activates a cell death cascade that is physiologically relevant, although the exact role of either the P2X- or MTX-induced cascade in normal cellular biology remains unknown.

  • Within the Signal Transduction category there were many genes associated with ligands, receptors and cytosolic factors (Figure 4Band Table 4).

  • Measurement of the apparent cytosolic free Ca 2+concentration


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