Words similar to cytoskeleton-associated
Example sentences for: cytoskeleton-associated
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This naturally raises the question of whether the cytoskeleton-associated transcripts are differentially expressed in promyelocyte and macrophage cells.
Among the subset of transcripts that were found to be enriched in the cytoskeleton fraction were several mRNA species, such as those encoding β-actin mRNA and ribosomal protein subunits, that have previously been shown to be cytoskeleton-associated in other cell types [ 13 16 ] . In addition, we identified approximately 20 new members of the cytoskeleton-associated transcriptome, the majority of which encode structural proteins known to interact directly with cytoskeleton filaments, such as spectrin and protein 4.1 (Table 1).
have previously monitored gene expression at various time points following phorbol ester-induced differentiation in the same HL-60 cell system [ 22 ] . Thirteen of the 22 transcripts we identified as cytoskeleton-associated were also assayed in that study by Tamayo, et al [ 22 ] . Only two of these transcripts-alpha spectrin and pim-1 were found to vary significantly, in that the expression of both genes was undetectable in the promyelocytic cell type and increased dramatically upon differentiation to the macrophage cell type.
Consistent with this hypothesis, the majority of cytoskeleton-associated transcripts identified in this study encoded proteins that modulate cytoskeleton structure or adhesion.
Upon centrifugation, the pellet, containing cellular matrix, was resuspended in high salt buffer to release cytoskeleton-associated components.
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