Words similar to adenylyl
Example sentences for: adenylyl
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A well established method for assessing G-protein activation by opioid receptors and characterizing activity of opioid ligands is measuring the extent of inhibition of forskolin-stimulated adenylyl cyclase (AC) activity [ 12 13 14 21 22 23 ] . Adenylyl cyclase has been implicated in playing a role in mediating the analgesic effect of opioid ligands through μ opioid receptors [ 24 25 26 27 28 ] . Thus, characterizing the ability of opioid ligands to inhibit cAMP production, such as described in this report, could be used as an index for assessing their activity as a correlate of their analgesic effects.
We have shown that δ-opioids increase the phosphorylation of ERKl/2 in HEK-293 cells when the mouse wild type (WT) δ-OR is stably expressed [ 11 13 ] . The concentrations required to observe DSLET-mediated ERK phosphorylation (EC 50 = 10 nM; Figure 1a) are consistent with those reported for other δ-OR-mediated events, including G protein activation and adenylyl cyclase inhibition [ 16 17 ] . Moreover, no change in total ERK expression was observed, concomitantly, with ERK phosphorylation in any of our experiments (data not shown).
Analogous active sites, that coordinate two metal ions, are observed in domains with similar activities, such as the classical adenylyl/guanylyl cyclases, family B DNA polymerases, pol-β fold nucleotidyl transferases, and triphosphatases or phosphoesterases of the HD and DHH superfamilies [ 11 15 19 20 ] . Consistent with these observations, both CyaB and ThTPase have been shown to require Mg 2+ions for their nucleotide cyclase and phosphatase activities [ 16 17 ] .
α s -GFP fusion proteins were assayed for the ability to stimulate adenylyl cyclase in response to luteinizing hormone (LH) receptor stimulation [ 38 ] . 10 6HEK 293 cells/60 mm-dish were co-transfected with 2 μg of plasmid DNA encoding the α s -GFP fusion protein, and 0.2 μg of plasmid DNA encoding the rat LH receptor in pCIS [ 39 ] , using 10 μL of Lipofectamine 2000.
In order to understand the evolutionary affinities and provenance of the CyaB adenylyl cyclase from Aeromonas hydrophila , we carried out database searches using sensitive sequence profile analysis methods.
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