Words similar to adenoviral

Example sentences for: adenoviral

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Adenoviral vectors
Replication-defective adenoviral vectors offer several advantages that make them attractive for gene delivery [ 3 4 5 6 ] . Adenoviral vectors are able to transduce proliferating or quiescent cells; they can accommodate dual, large insert sizes and can deliver additional genes by superinfection; and stable, high-titer viral stocks are easily generated [ 7 ] . Further, since the adenoviral genome is maintained extra-chromosomally, insertion site-dependent effects of the host genome are minimized, and exogenous genes can be reliably expressed at high levels.
The amount of adenoviral genomes per cell was determined by a multiplex real-time polymerase chain reaction [ 22 ] . Real-time PCR is based on the 5'-3' nuclease activity of AmpliTaq Gold polymerase, which allows it to cleave fluorogenic probes resulting in fluorescence.
The resulting biotinylated adenoviral vectors were incubated at 25°C for 2 h with gentle shaking in polystyrene wells, on which streptavidin is covalently immobilized (well diameter, 0.64 cm; Reacti-Bind Streptavidin Coated Polystyrene Strip Plates; Pierce Chemical) (5 × 10 6viral particles per well).
Thus, neither hCAR expression nor adenoviral transduction alters the naïve T cell's potential for Th1 or Th2 phenotype development.