Words similar to adenoviral
Example sentences for: adenoviral
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For adenoviral transduction, lymph nodes and spleen cells were isolated, treated with ACK lysing buffer for 3 min and washed in DMEM/10 mM HEPES (pH 7.2).
In this strategy, biotinylated adenoviral vectors are immobilized on the surfaces of streptavidin-coated microbeads with diameters up to a few micrometers [ 10 ] . For such adenovirus-microbead conjugates, great enhancement in transduction efficiency was seen with several different cell lines, including COLO205, the infection efficiency of which was not notably enhanced by adenoviral vectors immobilized on streptavidin-coated wells in the present study.
To examine the therapeutic effects of local and systemic IL-4 expression in established CIA, we have generated an adenoviral vector carrying the gene for murine IL-4 (Ad-mIL-4).
Similar to HT-29 cells, the transduction efficiency of adenoviral vectors for COLO205 is extremely poor [ 12 ] . The very low transduction efficiency of COLO205 by adenoviral vectors, used free in solution, was confirmed in our previous study [ 10 ] . When up to 2.5 × 10 7viral particles were added to each well at the immobilization step (or for transduction by free Ad5.CMV-LacZ), the infectivity of immobilized Ad5.CMV-LacZ was only slightly higher than free Ad5.CMV-LacZ in solution (data not shown).
One cell line is D-17, which is highly permissive to adenoviral infection when viral vectors are used free in solution [ 10 ] . The other cell line is C6 (rat glioma cells), which is less permissive to adenoviral infection [ 10 ] . For D-17 cells, the amount of infection increased steeply when the number of viral particles added to each well increased from 2.5 × 10 5to 2.5 × 10 6(Fig.
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