Words similar to adenoviral
Example sentences for: adenoviral
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For construction of the recombinant adenovirus containing the M6P/IGF2R-ribozyme (pAd-GFP/Rz-IGF2R), the segments containing the ribozymes were amplified by PCR and cloned into a pAdTrack-CMV vector and then recombined homologously with an adenoviral backbone pAdEasy 1 vector to generate pAd-GFP/Rz-IGF2R, following the protocol described by He et al [ 30 ] . The pAd-GFP/Rz-IGF2R carries both the Rz-IGF2R and GFP (as reporter) genes, each under the control of separate cytomegalovirus (CMV) promoters.
Unfortunately, naive lymphocytes are not susceptible to infection with adenoviruses due to lack of detectable expression of a viral receptor, such as the coxsackie/adenovirus receptor (CAR), which is required for efficient viral attachment [ 8 9 10 ] . The CAR protein contains two extra-cellular immunoglobulin-like (Ig) domains, the first of which appears sufficient for binding to the adenoviral fiber knob protein [ 11 12 ] . Expression of either human or murine CAR in various cell lines has been shown to facilitate gene transfer by recombinant human adenoviruses [ 8 9 10 13 ] . Stable transfection of transformed murine B and T cells lines with human CAR (hCAR) lacking its cytoplasmic domain rendered them susceptible to adenoviral transfection, establishing that lymphoid cells can be permissive hosts for adenoviral transduction and demonstrating that the cytoplasmic domain of CAR is not required for viral entry [ 13 ] . More recently, mice transgenic for full length [ 14 ] or truncated hCAR [ 15 ] have been described in which adenoviral transduction of primary lymphocytes has been demonstrated.
Adenoviral transduction
Construction of a recombinant adenoviral vector containing the M6P/IGF2R-ribozyme
To the biotin moieties on the solid surface, biotinylated adenoviral vectors were attached by using avidin as a molecular bridge between the biotin moieties on the solid surface and the viral surface.
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