Words similar to adenovirus
Example sentences for: adenovirus
How can you use “adenovirus” in a sentence? Here are some example sentences to help you improve your vocabulary:
The main organ infected by an adenovirus following iv injection is the liver [ 7 8 ] . Others and we find that the liver is producing 95-99% of the vector encoded protein after intravenous delivery of recombinant adenoviral vectors.
The volume of injection limits the quantity of adenovirus or plasmid that can be injected into the gastrocnemius of a mouse without overflow.
The adenoviral vector DNA were linerized with Pac I and transfected into the replication-permissive 293 cells (E1A transcomplementing cell line) using Lipofectamine (Life Technologies) to produce E1-deleted, replication-defective recombinant adenovirus as described previously [ 30 ] . Large-scale amplification of the recombinant adenovirus in 293 cells was followed by purification using a discontinuous CsCl gradient.
Ad-GFP, adenovirus carrying GFP gene; Ad-GFP/Rz-IGF2R, adenovirus carrying both ribozyme against M6P/IGF2R and GFP gene; GFP, green fluorescent protein; IGF-II, insulin-like growth factor II; M6P/IGF2R, mannose 6-phosphate/insulin-like growth factor II receptor; Rz, ribozyme, TGF-β transforming growth factor beta.
For construction of the recombinant adenovirus containing the M6P/IGF2R-ribozyme (pAd-GFP/Rz-IGF2R), the segments containing the ribozymes were amplified by PCR and cloned into a pAdTrack-CMV vector and then recombined homologously with an adenoviral backbone pAdEasy 1 vector to generate pAd-GFP/Rz-IGF2R, following the protocol described by He et al [ 30 ] . The pAd-GFP/Rz-IGF2R carries both the Rz-IGF2R and GFP (as reporter) genes, each under the control of separate cytomegalovirus (CMV) promoters.
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