Example sentences for: acetonitrile

How can you use “acetonitrile” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The active fractions were further purified using a Protein C4 Column (Vydac 25 cm × 4.6 mm ID) equilibrated with Solvent A (A= 5% acetonitrile; 0.1% heptafluorobutyric acid (HFBA) and resolution of peaks was accomplished using gradient elution of solvent B (B= 95% acetonitrile; 0.1% HFBA) reaching 60% in 70 min at a flow rate of 0.6 ml/min.

  • The freeze dried CM-fractions were resuspended in water and aliquots of 1 mg each were loaded onto the C4 reversed phase column equilibrated in Solvent A (A= 5% acetonitrile, 0.1% trifluroacetic acid, TFA).

  • 200 mL of 50% butanol:50% acetonitrile (v/v) was added and the mixture was vortexed for 60 seconds, followed by centrifugation for 2 minutes at 16,000 × g.

  • The C-4 column was equilibrated with 0.1% trifluoroacetic acid and eluted with an acetonitrile gradient (0-100%) run at 1%/ml/min.

  • The top organic layer was removed and diluted 1:5 in acetonitrile before injection onto the HPLC column.


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