Example sentences for: acetic

How can you use “acetic” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The gels were then incubated for 24 hours at 37°C in 50 mM Tris-HCl buffer, pH 7.4, containing 5 mM CaCl 2 . A duplicate negative control gel was incubated as described above but in 50 mM Tris-HCl, pH 7.4 containing 10 mM EDTA instead of CaCl 2 . The gels were fixed for 1 hour in 40% methanol /7% acetic acid and then stained in Coomassie Blue Solution (Sigma), followed by destaining in 10% methanol, 7% acetic acid.

  • Lipid A was extracted from isolated LPS preparations via acid hydrolysis [ 44 45 ] . Isolated LPS (25-30 mg) was suspended in 4 ml of a 1% acetic acid solution and heated in a water bath to 100°C under refluxing conditions for 3 hours.

  • The reaction was neutralized by the addition of 4 μl 5 M acetic acid.

  • Histone samples were resolved by electrophoresis using 15% acid-urea gel containing 36%w/v urea, 5%v/v acetic acid, 600 μl TEMED and 0.7 ml of 10% ammonium persulfate prepared as described elsewhere [ 27].

  • After trypsinization cells were centrifuged at 400 g for 5 min, swollen in 75 mM KCl for 10 min at 37°C, re-centrifuged and fixed in methanol:acetic acid (3:1) at 4°C, before dropping cells onto slides.

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