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Example sentences for: accumulation
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Catalytic activity of the Fhit ApppA hydrolase depends on His96 [ 14 16 ] . To test whether the active site of Hnt2 is necessary to control dinucleoside polyphosphate accumulation in vivo , wild-type and mutant alleles of HNT2 that differ at His109, corresponding to human Fhit His96, were expressed from the HNT2 promoter on plasmids (Table 3) in hnt2ΔADE2 strain BY71-6c.
Two moeB sequences with evidence for function include a knockout of the open reading frame encoding GenBank accession number BAA35521, which was shown to cause the accumulation of precursor Z in E. coli [ 13 ] and accession number Z95150, which was found to be located within a cluster of other MPT-Mo synthesis genes of Mycobacterium tuberculosis [ 14 ] . MSAEs from eukaryotic organisms have also been characterized: MOCS3 (GenBank accession number NM_014484) from humans and cnx 5 from Arabidopsis thaliana [ 15 ] , respectively, have been sequenced, and cnxF from Aspergillus nidulans [ 16 ] has been both sequenced and physiologically characterized.
Similar levels of signals were detected with and without IPTG (Figure 5c), thus indicating that lac repressor binding is inhibited by IPTG without change in protein accumulation levels.
COP activation can be monitored by the cellular accumulation of ethidium or propidium-based vital dyes, which are normally excluded from the cytoplasm, but gain access to cellular nucleotides via COP and exhibit an increase in fluorescence.
Results from the current study indicate that H 2 O 2 treatment of RPE could lead to the accumulation of 4-HNE protein adducts in the cytoplasm and the nuclei of these cells (Fig.
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