Words similar to ubiquitin-conjugating
Example sentences for: ubiquitin-conjugating
How can you use “ubiquitin-conjugating” in a sentence? Here are some example sentences to help you improve your vocabulary:
CUL1, in turn binds to SKP1, and together with HRT1, mediates the interaction with the ubiquitin-conjugating enzyme CDC34/UBC3.
Indeed, a similar explanation has been invoked to explain the presence of Hsp70 and ubiquitin in nuclear aggregates observed in cells expressing abnormal gene products with poly-Gln tracts, such as huntingtin [ 54 ] and ataxin-1 [ 59 ] . Based on reports that overexpression of chaperones [ 59 62 ] or a dominant-negative form of the ubiquitin-conjugating enzyme [ 63 ] can suppress the formation of poly-Gln protein aggregates, Kaytor and Warren [ 58 ] proposed a three-stage model wherein (i) molecular chaperones initially associate with the misfolded protein, (ii) failure to diminish the pool of misfolded protein leads to aggregation and ubiquitination and (iii) inefficient degradation of the aggregate by the proteasome is manifested by the accumulation of inclusion bodies.
The addition of a polyubiquitin chain to a protein requires the sequential action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2) and a ubiquitin-protein ligase (E3) [ 1 ] . E3 activities are thought to be essential because they are involved in substrate recognition.
UBC: ubiquitin-conjugating enzyme
Studies in budding yeast identified SCF Cdc4p, an E3 ubiquitin ligase complex that mediates the ubiquitylation of the CDK inhibitor Sic1p [ 3 4 ] . SCF Cdc4pconsists of at least four proteins: the cullin Cdc53p, the RING domain protein Hrt1p/Rbx1p/Roc1p, the adapter protein Skp1p, and Cdc4p (reviewed in [ 1 ] ). Cdc4p contains two sequence motifs, which are conserved in a wide variety of so-called F-box proteins: C-terminal WD-repeats that are involved in binding the substrate Sic1p in a phosphorylation-dependent manner, and a central F-box [ 5 ] that interacts with Skp1p [ 6 7 ] . Cdc53p in turn binds to Skp1p and Hrt1p/Rbx1p/Roc1p, which bridges Cdc53p with the ubiquitin-conjugating enzyme Cdc34p/Ubc3p [ 6 8 9 10 11 12 ] . In vitro reconstitution demonstrated that SCF Cdc4p, Cdc34p, E1, ubiquitin, and ATP are sufficient to mediate Sic1p polyubiquitylation [ 6 7 ] .
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