Words similar to ubiquitin-conjugating
Example sentences for: ubiquitin-conjugating
How can you use “ubiquitin-conjugating” in a sentence? Here are some example sentences to help you improve your vocabulary:
UBC: ubiquitin-conjugating enzyme
Studies in budding yeast identified SCF Cdc4p, an E3 ubiquitin ligase complex that mediates the ubiquitylation of the CDK inhibitor Sic1p [ 3 4 ] . SCF Cdc4pconsists of at least four proteins: the cullin Cdc53p, the RING domain protein Hrt1p/Rbx1p/Roc1p, the adapter protein Skp1p, and Cdc4p (reviewed in [ 1 ] ). Cdc4p contains two sequence motifs, which are conserved in a wide variety of so-called F-box proteins: C-terminal WD-repeats that are involved in binding the substrate Sic1p in a phosphorylation-dependent manner, and a central F-box [ 5 ] that interacts with Skp1p [ 6 7 ] . Cdc53p in turn binds to Skp1p and Hrt1p/Rbx1p/Roc1p, which bridges Cdc53p with the ubiquitin-conjugating enzyme Cdc34p/Ubc3p [ 6 8 9 10 11 12 ] . In vitro reconstitution demonstrated that SCF Cdc4p, Cdc34p, E1, ubiquitin, and ATP are sufficient to mediate Sic1p polyubiquitylation [ 6 7 ] .
For example, the ubiquitin-conjugating enzyme, which is important in protein degradation, identifies 6 unknown proteins out of 12, with 'C' or above confidence, which contain similar domains.
The addition of a polyubiquitin chain to a protein requires the sequential action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2) and a ubiquitin-protein ligase (E3) [ 1 ] . E3 activities are thought to be essential because they are involved in substrate recognition.
SCF complexes share homologues of the core components cullin 1 (CUL1), SKP1, and the RING domain protein HRT1/RBX1/ROC1, which associate with different F-box proteins and ubiquitin-conjugating enzymes [ 1].
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