Words similar to ubiquitylation

Example sentences for: ubiquitylation

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To determine whether SKP2 induces p27 ubiquitylation, cell lysate was prepared from MIB-exposed cells infected with Ad-SKP2 and Ad-SKP2-ΔF, and supplemented with phosphorylated p27, ATP, and ubiquitin.
Pop1p and Pop2p complexes were immunopurified with Myc antibodies and employed in in vitro ubiquitylation assays upon addition of E1, UBC3, ubiquitin, and ATP.
Cell lysates for ubiquitylation assays were prepared by hypotonic lysis on ice in 20 mM Hepes pH 7.5, 15 mM MgCl 2 , 5 mM KCl, 1 mM DTT, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 1 mM PMSF, 17 μg/ml aprotinin as described previously [ 30 ] . Lysates were cleared by centrifugation and used without prior freezing.
To exclude this possibility, we asked whether distinct SCF Pop1pand SCF Pop2pcomplexes bear ubiquitin ligase activity in vitro . To this end, we performed in vitro ubiquitylation assays.
Importantly, SKP2 overexpression is sufficient to reverse androgen-mediated p27 accumulation and induce p27 ubiquitylation (Fig.