Words similar to ubiquitination
Example sentences for: ubiquitination
How can you use “ubiquitination” in a sentence? Here are some example sentences to help you improve your vocabulary:
Post-transcriptional controls appear to be the major regulatory mechanisms that determine the levels of p27 Kip1in the cell [ 26 ] . These include ubiquitination and proteasomal degradation [ 27 ] and sequestration and mislocalization [ 28 ] of the p27 Kip1protein.
Because of its role in mediating the degradation of HY5, HYH, and possibly other transcription factors in Arabidopsis [ 4 5 ] and the presence of a RING finger domain and a WD-40 domain, AtCOP1 has been proposed to function as a RING-finger type ubiquitination E3 ligase, by recruiting E2 through its RING-finger domain and binding to the substrates through the WD-40 repeats [ 4 43 ] . This hypothesis is also supported by the findings about other negative regulators of plant photomorphogenesis.
In this study, we have demonstrated the mammalian COP1 is associated with ubiquitinated proteins and itself a substrate for ubiquitination in vivo.
A number of RING finger proteins have recently been shown to act as E3 ubiquitin ligases [ 24 ] . WD40 domains have also been frequently found in E3 complexes as substrate recruiting domains [ 25 26 27 28 ] . Despite compelling evidence from Arabidopsis implying that COP1 may mediate protein degradation [ 4 5 ] , no direct evidence of COP1 participating in ubiquitination has been reported.
As an initial step to study a possible role of mammalian COP1 in ubiquitination, we transiently expressed FLAG-tagged COP1 (FLAG-COP1) in human cell line 293 together with HA-tagged ubiquitin (HA-Ub), followed by immunoprecipitation with anti-FLAG antibodies.
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