Words similar to tne

Example sentences for: tne

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Membranes were probed with a 1:200-1:1000 dilution of antibodies at 4°C overnight, followed by three washes with TNE 50 plus 0.1% NP-40.
Total cellular protein was separated on 4-20% Tris-glycine gels (Novex, Inc.) and transferred to a polvinylidene difluoride (PVDF) membranes (Immobilon-P transfer membranes; Millipore Corp.) overnight at 0.08 A. Following the transfer, blots were blocked with 5% non-fat dry milk in 50 ml of TNE 50 (100 mM Tris-Cl [pH 8.0], 50 mM NaCl, 1 mM EDTA) plus 0.1% NP-40.
The next day, blots were incubated with 10 ml of 125I-protein G (Amersham, 50 μl/10 ml solution) in TNE 50 plus 0.1% NP-40 for 2 hrs at 4°C.
Finally, blots were washed twice in TNE 50 plus 0.1% NP-40 and placed on a PhosphorImager cassette for further analysis.