Words similar to phosphate
- phorbol
- phoretics
- phoretix
- phosphatase
- phosphatase-coupled
- phosphatase-labelled
- phosphatase-mediated
- phosphatased
- phosphatases
- phosphate
- phosphate-accepting
- phosphate-binding
- phosphate-buffered
- phosphate-conjugated
- phosphate-dehydrogenase
- phosphates
- phosphatidyl-
- phosphatidylcholine
- phosphatidylinositol-
- phosphatidylpropanol
Example sentences for: phosphate
How can you use “phosphate” in a sentence? Here are some example sentences to help you improve your vocabulary:
First, PfPP5 contained the catalytic core found in all Ser/Thr phosphatases of the PPP family, including the signature motifs such as GDXHGQ, GDFVDRG, RGNHE, HGLL, and SAPNYCD, to name a few [ 22 23 ] . Site-directed mutagenesis and structural studies in PP 1 and PPλ have previously established the roles of specific amino acid residues in these domains in the various aspects of catalysis, such as metal ion binding, phosphate recognition, and co-ordination of water molecules [ 24 25 26 ] .
Transfection of these cells with pAd-Lox-human TGF-β1 was performed by the calcium phosphate precipitation method with 3 μg pAd-Lox-human TGF-β1 construct digested with Sfi I and 3 μg ψ5 helper virus DNA.
zwf : zwf codes for glucose-6-phosphate dehydrogenase (G6PDH), the first enzyme in the oxidative branch of the PPP.
The samples were spotted along with pure phosphoinositol phosphate standards (Avanti Polar Lipids, Alabaster, AL, USA) onto 1.2% potassium oxalate impregnated TLC plates [ 45 ] . The chromatogram was developed in chloroform: methanol: water: aqueous ammonia (90:70:17:3) [ 46 ] . The incorporation of [ 32P] into phosphatidylinositol was quantitated by phosphorimaging using a Fuji BAS1000 phosphorimager.
Organic phosphate compounds are the central metabolites of all biological systems [ 1 2 ] . Some are the basic building blocks of nucleic acids, some like ATP and GTP, are additionally, cellular energy stores, others like cAMP or cGMP are messengers in signal transduction, and, yet others, such as FAD, NAD, thiamine phosphates and pyridoxal phosphate are cofactors for a range of enzymes [ 1 2 ] . Protein domains belonging to a relatively small set of structural folds are known to bind or catalyze reactions that utilize these organic phosphate compounds (see SCOP database: http://scop.mrc-lmb.cam.ac.uk/scop/) [ 3 4 ] . Several of these folds trace back to some of the earliest phases of the evolution of the protein world, and participate in a wide range of disparate biological functions in extant proteins [ 4 5 ] . Some folds, such as the P-loop fold, the Rossmann fold and the Hsp70-like fold, have been well studied, and comprise mainly of dedicated nucleotide binding or hydrolyzing proteins [ 6 7 8 9 ] . Others, such as the palm-domain, which is found in adenylyl cyclases and various nucleic acid polymerases, belong to more generalized protein folds that contain representatives with diverse biochemical activities [ 4 10 11 ] . Current availability of extensive genome sequence data, allows one to identify less numerous, nevertheless biological important organic phosphate-binding domains that may have previous eluded detection.
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