Example sentences for: phosphate

How can you use “phosphate” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The glyceraldehyde-3-phosphate dehydrogenase (GA3PD) gene was included as a reverse transcriptase polymerase chain reaction (RT-PCR) control and performed under similar conditions to normalize for the amount of RNA.

  • First-strand cDNA was purified from unincorporated amino allyl-dUTPs on QIAquick PCR purification columns (Qiagen, Valencia, CA) according to manufacturer's instructions, except that QIAquick wash buffer was replaced with 5 mM K +phosphate buffer (pH 8.5) containing 80% ethanol, and cDNA was eluted with 4 mM K +phosphate buffer (pH 8.5).

  • The remaining two essential gene products were in the pentosephosphate pathway ( tktAB, rpiAB ). The tktAB and rpiAB gene products were required for the synthesis of erythrose 4-phosphate in all phases (aromatic amino acid supplement required for the cultivation of tkt - E. coli mutant strains [ 27]).

  • Briefly, cryostat sections were fixed with acetone, dried for 30 minutes, and rinsed in phosphate buffered saline (PBS).

  • Animal fixation, tissue dissection, and microscopic analysis were performed as described previously [ 15 ] . In brief, mice were anesthetized and perfused with a solution of 4% paraformaldehyde and 2.5% glutaraldehyde in 0.1 M phosphate buffer, pH 7.6.


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