Example sentences for: phosphate

How can you use “phosphate” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Efficient tailing by terminal transferase requires the template DNA to be free of 3' phosphate groups, and is highly dependent on whether this template contains blunt- or overhanging-ends.

  • Separated proteins were electroblotted onto nylon membranes (Millipore Corp., Bedford, MA) which were then incubated for 2 h in a blocking solution containing 5% non-fat dry milk in phosphate buffered saline containing 0.02% Tween-20.

  • Another setting was an assay to probe activity of an enzyme that's of particular interest in Type 2 diabetes, called glucose-6-phosphate translocase.”

  • HeLa cells were transfected by calcium phosphate precipitation using 1 μg of plasmid expressing the full length receptor isoform and 1 μg of a ΔMTV-IR-CAT reporter gene, as previously described [ 22, 23].

  • Binding of β-glucuronidase was assayed as described previously [ 31 32 ] . Briefly, cells were permeabilized with 0.25% saponin in 50 mM Hepes (pH 7.0), 150 mM NaCl, 5 mM β-glycerophosphate, 0.5% human serum albumin, and 10 mM mannose-6-phosphate (M6P) for 30 minutes on ice.


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