Example sentences for: phosphate-buffered

How can you use “phosphate-buffered” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • BCIP = 5-bromo-4-chloroindol-3-yl phosphate; cDNA = complementary DNA; EGTA = (ethylene-bis [oxyethylenenitrilo])tetraacetic acid; CHTN = Cooperative Human Tissue Network; ELISA = enzyme-linked immunosorbent assay; MAP = microtubule associated protein; MES = 2-( N -morpholino)-ethane sulfonic acid; NBT = nitro blue tetrazolium; PBS = phosphate-buffered saline; PC-tubulin = MAP-free phosphocellulose-purified tubulin; PCR = polymerase chain reaction; PIPES = piperazine- N,N '-bis(2-ethanesulfonic acid); PVDF = poly(vinylidene difluoride).

  • Cells were harvested by centrifugation and washed once with phosphate-buffered saline (PBS) containing the following protease inhibitors: 2 mg/ml aprotinin, 2 mg/ml leupeptin, 2 mM phenylmethylsulfonyl fluoride (PMSF), and 0.2 mM pepstatin.

  • Following the block, cells were released by being washed twice with phosphate-buffered saline (PBS) and by the addition of complete medium.

  • Eighteen hours later, purified protein-free pGR(Ala)-GFP suspended in phosphate-buffered saline (0.

  • At the indicated times cells were harvested, washed twice with phosphate-buffered saline (containing 0.1% serum bovine albumin and 0.1% sodium azide), and incubated for 30 min at 4°C with specific monoclonal antibodies to either CD3, CD4 or CD8.


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