Words similar to phosphatase
Example sentences for: phosphatase
How can you use “phosphatase” in a sentence? Here are some example sentences to help you improve your vocabulary:
We have previously reported that mactinin promotes monocyte maturation, as measured by lysozyme secretion and tartrate-resistant acid phosphatase staining [ 7 ] . Here we show that 25 pM levels of mactinin promote monocytic maturation of the HL-60 leukemia cell line.
In light of the well-established growth-inhibitory effects exerted by increased levels of this phosphatase, it is reasonable to conclude that this activation of PTEN significantly contributes to the anchorage-dependent phenotype, i.e., to the inhibition of cell proliferation after detachment from matrix.
Several EF-hand proteins have been identified in the model plant Arabidopsis thaliana, including several CAMs [ 15, 16, 17, 18], a Ca 2+-binding protein (CaBP-22)[ 19], touch-induced proteins TCH2 and TCH3 [ 20], centrin [ 21], Ca 2+-dependent protein kinases (CPKs) [ 22], calcineurin B-like proteins/salt-overly-sensitive3 family (CBLs/SOS3) [ 23, 24], fimbrins [ 25], respiratory burst oxidase homologs (Rbohs) [ 26, 27], a phospholipase [ 28, 29], channel proteins [ 30, 31], a NAD(H)-dependent glutamate dehydrogenase [ 32], a protein phosphatase [ 33, 34], a NaCl-inducible protein [ 35], and a Ca 2+-binding protein in pollen [ 36].
For DNA fragments generated by ChIP (or blunt-end restriction) these limitations are effectively overcome by prior phosphatase treatment, by using recombinant terminal transferase enzyme, and by spiking a small quantity of terminating nucleotide into the tailing reaction.
Samples of known numbers of cells from the cell lines and recombinant, purified Tag prepared from a baculovirus lysate [ 40 ] were loaded on 10% polyacrylamide discontinuous mini-gels (Bio-Rad, Hercules, CA) and electrophoresed conventionally [ 41 ] . Gels were then electrophoretically blotted [ 42 ] onto PVDF membrane (Bio-Rad) without methanol for 15 min at 100 V. Tag was visualized by immunostaining with PAb416 and alkaline phosphatase conjugated secondary antibody (Sigma) using chemiluminescent detection with CDP-Star substrate according to the manufacturers directions (Tropix, Bedford, MA).
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