Words similar to phosphatase
Example sentences for: phosphatase
How can you use “phosphatase” in a sentence? Here are some example sentences to help you improve your vocabulary:
Determination of serum bilirubin, ALT, AST, alkaline phosphatase
Alkaline phosphatase activity was measured as follows: Cells were rinsed in cold TBS (Tris-buffered Saline), scrapped from the plate, homogenized in 0.05% Triton X-100 in Tris-HCl (pH 7.5) on ice, and the sample was centrifuged at 1200 rpm for 5 minutes at 4°C.
This GPCR-mediated regulation of ion channels also depends on the coordination and parallel regulation of protein tyrosine kinase and protein tyrosine phosphatase activities [ 24 ] . GPCRs can also interact with the growing family of PDZ domain-containing proteins [ 25 ] . All of these relationships are reflected in our study by the strong correlation between 7 transmembrane receptors (rhodopsin family) (PF00001), protein kinases (PF00069), protein tyrosine phosphatases (PF00102), ion transport proteins (PF00520), and PDZ domain proteins (PF00595).
PP2 is a serine/threonine phosphatase which dephosphorylates JNK during inflammatory cell signaling [ 19 ] , while MKP5 is a member of the dual specificity phosphatase family which selectively dephosphorylates stress activated MAP kinases including JNK [ 20 ] . The overexpression of these two phosphatases might contribute in part to the resistance of HL60/TLK286 by impairing the activity of JNK.
Protein phosphatase 5 has been implicated in the control of cell proliferation, in hormonal signal transduction, and in hsp90 chaperone function [ 3 11 12 13 14 15 16 17 ] . However little is known about the regulation of PP5 or its specific role in these pathways, and only one physiological substrate for PP5 has been identified to date, the apoptosis signaling kinase ASK 1 [ 13 ] . PP5 can be activated by lipid in vitro , and removal of the TPR domain or the C terminus generates an active, lipid insensitive form of the enzyme [ 18 19 20 21 ] . PP5 phosphatase activity is also altered by hCRY2 and a fragment of hsp90 in vitro [ 21 22 ] . These findings suggest that PP5 may be autoinhibited and that the binding of protein partners or lipids to the TPR domain could potentially control PP5 activity in cells, however no reports have yet documented how PP5 is regulated in vivo . The activity of PP5 homologs from cauliflower, T. brucei and P. falciparum is stimulated by polyunsaturated fatty acids in vitro [ 7 8 9 10 ] and removal of the TPR domain from P. falciparum PP5 increases activity to the same extent as lipid treatment [ 8 9 ] . This suggests that the TPR domain plays a similar inhibitory role for all PP5 homologs.
Loading...