Words similar to pgal

Example sentences for: pgal

How can you use “pgal” in a sentence? Here are some example sentences to help you improve your vocabulary:

To generate pGaL4-hCCR4, a fragment corresponding to the entire hCCR4 coding sequence (with the exception of the initiation codon) was obtained upon digestion of the KIAA1194 cDNA (accession number AB033020, gift from the Kazusa Research Institute, putative start codon -Met 19-positioned by homology with mCCR4) by Sph I and Msc I (nt 423 to 2172 in KIAA1194), and was inserted in-frame into the pGaL4PolyII vector opened by Xho I, after Klenow treatment of both vector and insert.
Construction of the deleted fusion protein in which four out the five hCCR4 leucine-rich repeats are removed (named pGal4-hCCR4(ΔLRR)) was performed in three steps.
These cells where in turn co-transfected with pSV40/Zeo2 (Invitrogen) and pG5-luc (Promega), encoding firefly luciferase or pGAL4-SEAP and MBT-2 cells were selected in 400 μg/ml Zeocin and Panc02 cells in 500 μg/ml Zeocin.
The pGAL4 and pG5CAT plasmids were obtained from the Mammalian MATCHMAKER Two-Hybrid Assay Kit (CLONTECH).
As a control, pGal4-hCCR4 alone, or in association with the pVP16-none plasmid, had no effect.