Example sentences for: pgal

How can you use “pgal” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 4B, co-expression of pGal4-hCCR4 with either pVP16-hCAF1 or pVP16-hPOP2 elicited a significant increase (> 35 fold) in pG4-TK-Luc expression, thus demonstrating association between hCCR4 and either hCAF1 or hPOP2.

  • Construction of the deleted fusion protein in which four out the five hCCR4 leucine-rich repeats are removed (named pGal4-hCCR4(ΔLRR)) was performed in three steps.

  • Finally, the deleted open reading frame-containing fragment was excised using Sph I and Msc I, and inserted into the pGaL4PolyII vector opened by Xho I, after Klenow treatment of both vector and insert.

  • The pGAL4 and pG5CAT plasmids were obtained from the Mammalian MATCHMAKER Two-Hybrid Assay Kit (CLONTECH).

  • Cell clones were transiently transfected using Superfect reagent with 1 μg per well of 6 well plate with either pFR-luc (Promega) or pGAL4-SEAP (secreted alkaline phosphatase) (provided by Dr. R. Herzig, Rheogene Inc.) and 3 μg carrier DNA (pLITMUS, New England Biolabs).


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