Words similar to pgal
Example sentences for: pgal
How can you use “pgal” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cell clones were transiently transfected using Superfect reagent with 1 μg per well of 6 well plate with either pFR-luc (Promega) or pGAL4-SEAP (secreted alkaline phosphatase) (provided by Dr. R. Herzig, Rheogene Inc.) and 3 μg carrier DNA (pLITMUS, New England Biolabs).
4B, co-expression of pGal4-hCCR4 with either pVP16-hCAF1 or pVP16-hPOP2 elicited a significant increase (> 35 fold) in pG4-TK-Luc expression, thus demonstrating association between hCCR4 and either hCAF1 or hPOP2.
MBT-2 cells, a mouse urothelial cancer cell line, or Panc02, a mouse ductal adenocarcinoma, were transfected by electroporation with a mixture of the four plasmids; the two components of the gene expression system, pVP16-mRXR and pGAL4-EcR; the indicator and selection plasmid, pGAL4-EGFP-SV40-neo; and the internal control plasmid, pRL-TK.
Finally, the deleted open reading frame-containing fragment was excised using Sph I and Msc I, and inserted into the pGaL4PolyII vector opened by Xho I, after Klenow treatment of both vector and insert.
As a control, pGal4-hCCR4 alone, or in association with the pVP16-none plasmid, had no effect.