Example sentences for: pgal

How can you use “pgal” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 4B, co-expression of pGal4-hCCR4 with either pVP16-hCAF1 or pVP16-hPOP2 elicited a significant increase (> 35 fold) in pG4-TK-Luc expression, thus demonstrating association between hCCR4 and either hCAF1 or hPOP2.

  • The vector pGAL4-SEAP containing the GAL4 response element and the coding region for the SEAP protein, a unique, second reporter gene, was also used.

  • MBT-2 cells, a mouse urothelial cancer cell line, or Panc02, a mouse ductal adenocarcinoma, were transfected by electroporation with a mixture of the four plasmids; the two components of the gene expression system, pVP16-mRXR and pGAL4-EcR; the indicator and selection plasmid, pGAL4-EGFP-SV40-neo; and the internal control plasmid, pRL-TK.

  • To generate pGa14-m.nocturnin, a fragment from the m.nocturnin cDNA [ 21 ] corresponding to the entire coding sequence (with 9 amino acids upstream of the initiation codon) was excised from pGEX-3X upon Sma I and Dra I digestion, and was inserted into the pGaL4PolyII vector opened by Bam Hl, after Klenow treatment of both vector and insert.

  • The inducible reporter gene plasmids, pG5-luc and pGAL4-SEAP, were transfected into the Panc02+RHeoSwitch-3 and -4 cells and stable transfectants were isolated as they were into the MBT-2+RHeoSwitch cells.


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