Words similar to pgal
Example sentences for: pgal
How can you use “pgal” in a sentence? Here are some example sentences to help you improve your vocabulary:
4B, co-expression of pGal4-hCCR4 with either pVP16-hCAF1 or pVP16-hPOP2 elicited a significant increase (> 35 fold) in pG4-TK-Luc expression, thus demonstrating association between hCCR4 and either hCAF1 or hPOP2.
Construction of the deleted fusion protein in which four out the five hCCR4 leucine-rich repeats are removed (named pGal4-hCCR4(ΔLRR)) was performed in three steps.
Finally, the deleted open reading frame-containing fragment was excised using Sph I and Msc I, and inserted into the pGaL4PolyII vector opened by Xho I, after Klenow treatment of both vector and insert.
The pGAL4 and pG5CAT plasmids were obtained from the Mammalian MATCHMAKER Two-Hybrid Assay Kit (CLONTECH).
Cell clones were transiently transfected using Superfect reagent with 1 μg per well of 6 well plate with either pFR-luc (Promega) or pGAL4-SEAP (secreted alkaline phosphatase) (provided by Dr. R. Herzig, Rheogene Inc.) and 3 μg carrier DNA (pLITMUS, New England Biolabs).