Example sentences for: pgal

How can you use “pgal” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The GAL4 fusion plasmids were obtained by subcloning the appropriate cDNAs into the pGaL4PolyII plasmid [ 24 ] , in-frame with the yeast GAL4 binding domain coding sequence.

  • 4B, co-expression of pGal4-hCCR4 with either pVP16-hCAF1 or pVP16-hPOP2 elicited a significant increase (> 35 fold) in pG4-TK-Luc expression, thus demonstrating association between hCCR4 and either hCAF1 or hPOP2.

  • Cell clones were transiently transfected using Superfect reagent with 1 μg per well of 6 well plate with either pFR-luc (Promega) or pGAL4-SEAP (secreted alkaline phosphatase) (provided by Dr. R. Herzig, Rheogene Inc.) and 3 μg carrier DNA (pLITMUS, New England Biolabs).

  • pGAL4-SRF was constructed as previously described [ 23].

  • The vector pGAL4-EGFP was constructed from pCI-neo (Promega) by replacing the CMV promoter and enhancer with a fragment from pG5-luc (Promega) containing the 5 × GAL4 recognition sequences and the minimal promoter from the adenoviral late promoter and the EGFP gene from pEGFP (Clontech).

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