Words similar to pgal

Example sentences for: pgal

How can you use “pgal” in a sentence? Here are some example sentences to help you improve your vocabulary:

To generate pGa14-m.nocturnin, a fragment from the m.nocturnin cDNA [ 21 ] corresponding to the entire coding sequence (with 9 amino acids upstream of the initiation codon) was excised from pGEX-3X upon Sma I and Dra I digestion, and was inserted into the pGaL4PolyII vector opened by Bam Hl, after Klenow treatment of both vector and insert.
The vector pGAL4-EGFP was constructed from pCI-neo (Promega) by replacing the CMV promoter and enhancer with a fragment from pG5-luc (Promega) containing the 5 × GAL4 recognition sequences and the minimal promoter from the adenoviral late promoter and the EGFP gene from pEGFP (Clontech).
MBT-2 cells, a mouse urothelial cancer cell line, or Panc02, a mouse ductal adenocarcinoma, were transfected by electroporation with a mixture of the four plasmids; the two components of the gene expression system, pVP16-mRXR and pGAL4-EcR; the indicator and selection plasmid, pGAL4-EGFP-SV40-neo; and the internal control plasmid, pRL-TK.
Cells were simultaneously co-transfected by electroporation (Gene Pulser, Bio-Rad Inc.) with pGAL4-EcR, pVP16-mRXR, pRL-TK (Renilla luciferase) and pGAL4-EGFP.
The complete hCCR4 cDNA was obtained (KIAA1194 cDNA, Kazusa DNA Research Institute), and vectors were constructed to express hCCR4 fused to the DNA binding domain of the yeast GAL4 transcription factor (pGal4-hCCR4) (Fig.