Words similar to ph
Example sentences for: ph
How can you use “ph” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cleavage reactions contained 30 nM substrate RNA, increasing amounts (60 nM) of ribozyme, 20 mM MgCl 2 and 20 mM Tris-HCl (pH 8.0), 1 μl DTT, and 1 μl RNA inhibitor in a final volume of 10 μl.
Staining was stopped by a 10 min wash in Tris/EDTA (10/1 mM, ph 8.0), and non-specific staining was removed by 1 hour in 95% EtOH.
Cells were lysed in a buffer containing 50 mM Tris-HCl (pH 7.6), 5 mM EDTA, 150 mM NaCl, 0.5 % NP-40, and 0.5% Triton X-100 containing 1 μg/ml leupeptin, aprotinin and antipain; 1 mM sodiumorthovanadate; and 0.5 mM PMSF (all reagents were obtained from Sigma Chemical Co.).
0. Sensitivity was reduced at < pH7.
The reaction cocktail contained 1 ng EST plasmid DNA, 1.7 mM MgCl 2 , 1x reaction buffer (50 mM Tris pH 8.5, 20 mM KCl), and 0.1 units of Taq polymerase (GibcoBRL, Gaithersburg, MD).