Words similar to ph
Example sentences for: ph
How can you use “ph” in a sentence? Here are some example sentences to help you improve your vocabulary:
For nested amplification, 1 μl of amplified PCR reaction mixture was added to a second PCR reaction mixture (50 μl of 10 mmol/l Tris-HCl [pH 9.0], 50 mmol/l KCl, 1.5 mmol/l MgCl 2 , 0.01% Triton-X, 200 μmol/l dNTP, and 1.25 U Taq DNA polymerase).
Samples were covered with labelling reaction mixture (50 mM Tris, pH 7.5; 5 mM MgCl
The pellets from the ammonium sulfate fractionation were resuspended in 60 ml of buffer B (20 mM HEPES pH 8.5 4°C, 1 mM EDTA, and 0.1% 2-mercaptoethanol), desalted on a G-25 sephadex column equilibrated with buffer B, then subjected to sequential chromatography on a 5 ml HiTrap Heparin column (Pharmacia), a 5 ml HiTrap Q column (Pharmacia) and a HiTrap SP column (Pharmacia) respectively.
The structural interactions that underlie tetramerization of KcsA on gels are disrupted only by heating the sample and by pH 12 [ 31 ] , treatments that had no effect on the dimerization of VR1 we observed (data not shown).
The two amino-acid side chains differ in shape, hydrogen-bonding capability and degree of positive charge (arginine always being positively charged at physiological pH, histidine only having a positive charge about half the time).
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