Words similar to pgminvrpt
Example sentences for: pgminvrpt
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0 to generate construct PGMinvrpt (Figure 2c).
For example, PGMinvrpt(3)76 is PGMinvrpt construct inserted on chromosome 3, independent transgenic line 76.
To determine whether altered Pgm gene expression could affect lifespan, mean lifespan was assayed in multiple PGMsense and PGMinvrpt transgenic lines with or without DOX treatment.
The PGMinvrpt construct yielded decreases in PGM activity of as much as 50%; however, the effect was quite variable from experiment to experiment (Table 1).
The PGMinvrpt construct was made as follows: because the final insert would be a large inverted repeat, Pgm Swas PCR-amplified in two pieces, in opposite orientations.