Words similar to pgk
Example sentences for: pgk
How can you use “pgk” in a sentence? Here are some example sentences to help you improve your vocabulary:
acnAB, gapAC, gltA, icdA, pgk, rpiAB, tktAB ). Specifically, the gltA -, icdA -, and acnAB - in silico deletion strains were unable to produce one biosynthetic precursor (α-ketoglutarate, Table 1), and retained the capability to synthesize the remaining biosynthetic precursors and cofactors nearly equivalent to the wild-type.
Cre-mediated deletion of the pgk promoter results in the cells reverting to G418 sensitivity while retaining the Neo gene.
The pgk promoter was deleted from 25% of transfected Hnf3αloxPNeo ES cells, using the approach described in materials and methods.
A Xho1/HindIII (blunt) cassette containing the Tn5 neomycin phosphotransferase ( Neo )gene, that could confer resistance to G418 and whose expression in mammalian cells was directed by a phosphoglycerate kinase-1 ( pgk) promoter, was introduced into an XbaI (blunt) site between the Hnf3α genomic sequences [ 16, 25].
The herpes simplex virus thymidine kinase ( hsv-TK ) gene, whose expression was also regulated by the pgk promoter, was introduced adjacent to the Hnf3α 5' arm of homology to provide negative selection in the presence of gancylovir.