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Example sentences for: pgk

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acnAB, gapAC, gltA, icdA, pgk, rpiAB, tktAB ). Specifically, the gltA -, icdA -, and acnAB - in silico deletion strains were unable to produce one biosynthetic precursor (α-ketoglutarate, Table 1), and retained the capability to synthesize the remaining biosynthetic precursors and cofactors nearly equivalent to the wild-type.
4 kb cassette containing the 5' end of Neo coding sequence accompanied by the pgk promoter with flanking loxP elements was introduced into the SphI site (blunt) of pNEB193 (New England Biolabs).
ES cell lines from which the pgk promoter had been deleted were named Hnf3αΔpgk-Neo to indicate this event.
The pgk promoter was deleted from 25% of transfected Hnf3αloxPNeo ES cells, using the approach described in materials and methods.
Southern blot analysis (fig 3) shows that deletion of the pgk promoter in Hnf3αloxPNeo cells generated a predicted 3.5 kb HindIII/EcoRV fragment that was detected by probe A. Moreover, Fig 3cshows that deletion of the pgk promoter resulted in the Hnf3αloxPNeo cells reverting to G418-sensitivity because the promoter is necessary for Neo expression.