Words similar to pgk

Example sentences for: pgk

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Furthermore, the essential glycolytic gene products ( pgk, gapAC ) were required for the synthesis of oxaloacetate, succinyl-CoA, α-ketoglutarate, pyruvate, phosphoenolpyruvate (PEP), and 3-phosphoglycerate within all conditions, and were unable to synthesize all biosynthetic precursors under anaerobic growth conditions.
Southern blot analysis (fig 3) shows that deletion of the pgk promoter in Hnf3αloxPNeo cells generated a predicted 3.5 kb HindIII/EcoRV fragment that was detected by probe A. Moreover, Fig 3cshows that deletion of the pgk promoter resulted in the Hnf3αloxPNeo cells reverting to G418-sensitivity because the promoter is necessary for Neo expression.
In step 2 a Cre-expression plasmid is transiently introduced into the pgk/loxP-Neo targeted ES cells.
The 3' arm of homology consisted of the 5' end of Neo coding sequence accompanied by the pgk promoter with flanking loxP elements.
The expression of Cre recombinase mediated recombination between the loxP elements that flanked the pgk promoter.