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Example sentences for: pgmy
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The PGMY09/11 L1 consensus HPV L1 primers and reverse line blot hybridization system was used to detect 38 genital HPV types as described earlier [ 27 28 ] . Appropriate control experiments were set up using bulk master mix components to eliminate potential contamination.
PCR amplification with PGMY09/11 L1 consensus HPV L1 primers followed by reverse line blot hybridization was used to detect 38 genital HPV types as described earlier [ 53 54 ] . Appropriate control experiments were set up using bulk master mix components to eliminate potential contamination.
HPV DNA was amplified with the PGMY09/PGMY11 primer system that uses a multiplex format to amplify both HPV and β-globin in the same tube [ 9 ] . To allow for strand separation, only the downstream primers designated PGMY11 and one β-globin primer were biotinylated (Table 1).
For these experiments, we designed capture (Table 1) and signal (Table 2) probes to hybridize specifically to the exon sequence of β-globin and to HPV sequences within the 450-bp L1 region amplified by the PGMY primer set.