Words similar to pfu
Example sentences for: pfu
How can you use “pfu” in a sentence? Here are some example sentences to help you improve your vocabulary:
A primer complementary to the19 bp Tn 5 ME (5'-CTGTCTCTTATACACATCT-3') was used to amplify the transposons (1 cycle at 95°C for 3:30 min., 24 cycles of 95°C for 30 sec 47°C for 30 sec 72°C for 1 min., 1 cycle at 72°C for 5 min.) with Pfu polymerase (Stratagene, La Jolla, CA).
All viruses yielded similar PFU/ml (data not shown).
Using an Advantage-GC cDNA PCR polymerase (Clontech) and Pfu DNA polymerase (Stratagene), PCR was carried out with primers specific to the exon and/or intron of DMN sequence (Table 1) under the following conditions: 94°C for 1 min followed by 35 cycles of 94°C for 1 min, 57-59 °C for 1 min, 72°C for 3 min and a final extension time of 10 min at 72°C.
The RT reaction was performed using AMV reverse transcriptase, and all PCRs were performed with a 15:1 mixture of Taq:Pfu polymerase.
This number was calculated as follows: (number of plaques 270) × (dilution factor 10) × (total packaging volume 500 μl) / (total number of mg packaged 8.75 × 10 -5) × (number of μl packaged 1) = 1.542 × 10 10plaque-forming units (PFU) per mg or 1.35 × 10 6PFU per packaging reaction.