Words similar to pfu
Example sentences for: pfu
How can you use “pfu” in a sentence? Here are some example sentences to help you improve your vocabulary:
PCR amplification was performed in a 50 ul volume with the PfuTurbo Hotstart DNA Polymerase system (Stratagene) and included 0.2 mM dNTPs, 100 ng each primer, 2.5 units PfuTurbo DNA polymerase, 100 ng genomic DNA, and 1X cloned Pfu DNA polymerase reaction buffer.
92 log 10 PFU/ml) was statistically significant (p = 0.049).
45 log 10 PFU/ml) and FCV-treated (2.
A primer complementary to the19 bp Tn 5 ME (5'-CTGTCTCTTATACACATCT-3') was used to amplify the transposons (1 cycle at 95°C for 3:30 min., 24 cycles of 95°C for 30 sec 47°C for 30 sec 72°C for 1 min., 1 cycle at 72°C for 5 min.) with Pfu polymerase (Stratagene, La Jolla, CA).
Following isolation of pure plaques, the insert DNAs were prepared by PCR methods with the following conditions: 100 μl reactions containing 1X Pfu buffer (20 mM Tris-Cl, pH 8.75, 10 mM KCl, 2 mM MgSO 4 , 10 mM (NH 4 ) 2 SO 4 , 0.1% v/v Triton X-100, 100 μg/ml bovine serum albumin), 1 μM λgt11 forward and reverse primers (Promega, Madison, WI), 0.2 mM deoxyribonucleotides, and 5 Units of recombinant Pfu DNA Polymerase (Stratagene, La Jolla, CA).