Words similar to pfu

Example sentences for: pfu

How can you use “pfu” in a sentence? Here are some example sentences to help you improve your vocabulary:

A cDNA that encodes a hemagglutinin-tagged NPM3 protein was produced by PCR under the following conditions: 100 μl reactions with 1X Pfu buffer, 0.2 mM deoxyribonucleotides, 1 μM HA-F primer (5'-AAA GAA TTC AGC ATG TAC CCA TAC GAC GTC CCA GAC TAC GCC GCC GCC GGT ACT GCA GCT GCC-3'), 1 μM NPM3 -R2 primer (5'-AAA GAA TTC CTA GGG CCT GCC CCC CTG CTT TTT GGC AGG AAG GAT GGG-3'), 1 ng of human NPM3 cDNA insert, and 2.5 Units of recombinant Pfu DNA Polymerase.
We used genomic DNA for a template, and for each mutant transcribed the complete ftsZ sequence (adding NdeI and BamHI cloning sites at the ends) using error-correcting Pfu polymerase [ 37] and outward-facing primers containing the desired mutations.
Virus stocks were prepared by inoculating cells at 0.01 plaque-forming units per MRC-5 cell (PFU/cell).
We were able to obtain from the dilution gradient filter spiking experiments a positive band from a 1:500,000 dilution of virus, which translates into a detected amount of approximately 0.77 TCID 50 or 0.39 PFU's per filter.
All viruses yielded similar PFU/ml (data not shown).