Example sentences for: pgbd-ctop

How can you use “pgbd-ctop” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • pGBD-cTOP1 was co-transfected with the HeLa cell library (Clontech, Cat.

  • To construct the cTOP1 expression vector (pDBleu-TOP1c), cTOP1 was excised from pGBD-cTOP1 with EcoRI and BamHI and inserted into the same sites in pDBleu (ProQuest, Life Technologies).

  • To construct the bait expression plasmid (pGBD-cTOP1), the core domain of TOP1 (cTOP1, amino acids 198-651) was amplified by PCR (sense primer: 5'-CGGAATTCGAAGAGGAACAGAAGTGGAAATGG-3'; antisense primer: 5'-CGGGATCCTTAAATCTTAGTTTGCAAGTTCATCAT-3') and inserted into the vector pGBD (Clontech) cut with EcoRI and BamHI.

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