Words similar to pgbt

Example sentences for: pgbt

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Y190 transformants containing pGBT9/proHB-EGFEx and pGAD10/cv-1 library were plated onto Trp -Leu -His -agar plates.
The pGBT9/proHB-EGFEx construct was used as the bait construct in the yeast two-hybrid screen to identify extracellular proteins that interact with the extracellular domain of proHB-EGF.
A negative control of Y187 or Y190 containing both plasmid vectors, pGBT9 and pGAD424, was included in all β-galactosidase assays.
The PCR product was digested with Eco RI and Bam HI and ligated into the Eco RI and Bam HI restriction sites of pGBT9 and pGAD424 to produce constructs pGBT9/proHB-EGFEx and pGAD424/proHB-EGFEx, respectively.
The plasmid vectors, pGBT9, pGAD424, pTD1, pVA3, the monkey cv-1 cDNA library in pGAD10, and the S. cerevisiae strains Y187 and Y190 were obtained from Clontech.