Words similar to pgbt

Example sentences for: pgbt

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To test whether the fibulin-1C interaction with proHB-EGF maps to the heparin-binding region, a construct, pGBT9/prodelHB-EGF, containing amino acid residues Asp 106-His 159(the entire EGF like region of proHB-EGF and 18 out of 32 residues of the heparin-binding region) [ 2 ] was used in the yeast two-hybrid system (see materials and methods).
The pGBT9/proHB-EGFEx construct was used as the bait construct in the yeast two-hybrid screen to identify extracellular proteins that interact with the extracellular domain of proHB-EGF.
Each construct from the cDNA library that tested positive for both lacZ and his reporter gene expression was also tested for the ability to activate directly β-galactosidase/ his reporter gene transcription without the presence of the bait construct, pGBT9/proHB-EGFEx.
The PCR product was digested with Eco RI and Bam HI and ligated into the Eco RI and Bam HI restriction sites of pGBT9 to produce construct pGBT9/prodelHB-EGF.
The PCR product was digested with Eco RI and Bam HI and ligated into the Eco RI and Bam HI restriction sites of pGBT9 and pGAD424 to produce constructs pGBT9/proHB-EGFEx and pGAD424/proHB-EGFEx, respectively.