Words similar to pgbt

Example sentences for: pgbt

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The PCR product was digested with Eco RI and Bam HI and ligated into the Eco RI and Bam HI restriction sites of pGBT9 and pGAD424 to produce constructs pGBT9/proHB-EGFEx and pGAD424/proHB-EGFEx, respectively.
Construct pGBT9/prodelHB-EGF was unable to interact with pGAD424/Fib-1C EGF#5-COOH (data not shown).
The results of screening the monkey cv-1 cDNA library with the bait plasmid pGBT9/DTREx were somewhat surprising in that no portions of the CD9 protein were represented among the proteins found to interact with the extracellular domain of proHB-EGF.
To test whether the fibulin-1C interaction with proHB-EGF maps to the heparin-binding region, a construct, pGBT9/prodelHB-EGF, containing amino acid residues Asp 106-His 159(the entire EGF like region of proHB-EGF and 18 out of 32 residues of the heparin-binding region) [ 2 ] was used in the yeast two-hybrid system (see materials and methods).
The plasmid vectors, pGBT9, pGAD424, pTD1, pVA3, the monkey cv-1 cDNA library in pGAD10, and the S. cerevisiae strains Y187 and Y190 were obtained from Clontech.