Example sentences for: pgem-t

How can you use “pgem-t” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The primer names refer to their position relative to the LSU rRNA of E. coli . PCR amplification products were cloned in the pGEM-T (Promega) vector and sequenced over both strands.

  • At first, we made degenerate deoxyoligonuclotide primers corresponding to the conserved peptide sequences GDXHGQ and GDXVDRG of PPs [ 17 ] . An approximately 120 bp PCR product, obtained by using these primers with the Pf 3D7 genomic DNA as template, was cloned in the pGEM-T vector (Promega) using the T/A cloning strategy.

  • 1) eventually produced a RT-PCR product that was cloned into pGEM-T vector and sequenced.

  • The products were cloned into pGEM-T and confirmed by sequencing.

  • The various products were gel-purified and cloned in pGEM-T by "TA cloning" (Promega, WI).

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