Words similar to pgad

Example sentences for: pgad

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The constructs were sequenced as described above using primers pGAD10SEQ and pGAD10cSEQ.
The plasmid vectors, pGBT9, pGAD424, pTD1, pVA3, the monkey cv-1 cDNA library in pGAD10, and the S. cerevisiae strains Y187 and Y190 were obtained from Clontech.
The construct was sequenced as described above using the primers pGAD10SEQ and pGAD10cSEQ.
The PCR product was digested with Eco RI and Bam HI and ligated into the Eco RI and Bam HI restriction sites of pGBT9 and pGAD424 to produce constructs pGBT9/proHB-EGFEx and pGAD424/proHB-EGFEx, respectively.
In constrast, the construct, pGAD424/Fib-1C EGF#1-5, that contained calcium-binding type II EGF-like modules #1-5 fused to the GAL4 activation domain (see Fig.