Words similar to pgad

Example sentences for: pgad

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The construct was sequenced as described above using the primers pGAD10SEQ and pGAD10cSEQ.
The PCR product was digested with Eco RI and Bam HI and ligated into the Eco RI and Bam HI restriction sites of pGBT9 and pGAD424 to produce constructs pGBT9/proHB-EGFEx and pGAD424/proHB-EGFEx, respectively.
The PCR products were digested with Eco RI and Sal I and ligated into the Eco RI and Sal I restriction sites of pGAD424 to produce constructs pGAD424/Fib-1C EGF#1-5 and pGAD424/Fib-1C EGF#5-COOH respectively.
Y190 transformants containing pGBT9/proHB-EGFEx and pGAD10/cv-1 library were plated onto Trp -Leu -His -agar plates.
In constrast, the construct, pGAD424/Fib-1C EGF#1-5, that contained calcium-binding type II EGF-like modules #1-5 fused to the GAL4 activation domain (see Fig.