Words similar to pdh

Example sentences for: pdh

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Amplification of the housekeeping gene G3PDH served as a control for RNA integrity.
From each animal, quantitative real-time PCR determinations of 5 target genes ( cry1,2, per1,2 , and dbp ) and a reference cDNA (glyceraldehyde-3-phosphate dehydrogenase, g3pdh ) were made from the cortex, basal forebrain, and hypothalamus (primer/probe sequences available upon request).
Samples from the G3PDH gene fragment amplifications using the subtracted and unsubtracted cDNA pools were analyzed after 15, 20, 25 and 30 cycles of PCR.
For each experimental sample, the amount of the target and g3pdh reference was determined from the standard curve (range of 0.2-200 ng total RNA) measured in the same assay.
, less than 100 nM) can selectively suppress HIV-1 transcript elongation without altering cellular transcripts in cell types of various lineages, including podocytes [ 7 29 30 ] . Cyclin D 1 transcript levels were followed before, during, and after treatment of confluent wild-type or HIV-1 infected podocytes with 50 nM flavopiridol, a dose that suppresses HIV-1 transcription by 70% [ 7 ] . Analysis of HIV-1, cyclin D 1 , and glyceraldehyde-3-phosphate dehydrogenase (G3PDH) transcript levels in podocytes treated with flavopiridol for 2 days, followed by drug washout for 1 day, showed that cyclin D 1 expression paralleled HIV-1 expression in infected podocytes before, during and after suppression of HIV-1 genes (Figure 4).