Words similar to pdh
Example sentences for: pdh
How can you use “pdh” in a sentence? Here are some example sentences to help you improve your vocabulary:
From each animal, quantitative real-time PCR determinations of 5 target genes ( cry1,2, per1,2 , and dbp ) and a reference cDNA (glyceraldehyde-3-phosphate dehydrogenase, g3pdh ) were made from the cortex, basal forebrain, and hypothalamus (primer/probe sequences available upon request).
RNA and protein samples were analyzed as follows: Podocyte RNA (10 μg) was analyzed by northern blot using probes for HIV-1 nef-LTR [ 7 ] , mouse cyclin D 1 (cDNA generated by RT-PCR from mouse podocytes and verified by sequence analysis), synaptopodin [ 7 ] , and G3PDH.
The tester cDNA was also amplified with G3PDH 3' primer and an adaptor-specific primer, which yields a fragment that is 200 bp bigger than the fragment generated with the gene-specific primers.
Following Northern hybridization with these probes, the blots were stripped and re-probed with a mouse G3PDH probe for normalization purposes.
Amplification of the housekeeping gene G3PDH served as a control for RNA integrity.
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