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Initially, to determine whether sarcospan was contained on YACs or BACs spanning the CFEOM1 critical region, YACs 766h7, 762e1, 951h6, 813h9, 782e8, 813g11, 832f4, 887g10, 916c8, 936f5, 946d5, 852c3, 723h3, 906f9, 798b12, 958b2, 943f6, 957b8, and 973h3 [ 2] and BACs B455J16, B50I19, B459A22, B392F18, B193D8, B855O3, B460N10, B351C12, B56H16, B937H2, B1035D8, B251K10, B152M7, B396F22, B45D10, B471G7, B520I18, and B367O10 (AECOM Genome Center Home Page chromosome 12 maps http://sequence.aecom.yu.edu/chr12/QARM1.pdfand http://sequence.aecom.yu.edu/chr12/Parm2.pdf) were tested.
5 cM (Chromosome 12p map page 3 (search for D12S1584) http://sequence.aecom.yu.edu/chr12/Parm2.pdfand Chromosome 12q map page 1 (search for D12S1668) http://sequence.aecom.yu.edu/chr12/QARM1.pdf), thus placing sarcospan outside of the CFEOM1 critical region.