Words similar to gat
- g-g---
- g-moms
- g-n-t-r
- g-zipped
- g. trent
- gas
- gasb
- gasb--governmental
- gasoline
- gassoc
- gastritis
- gastrocnemius
- gastroenterology
- gastrointestinal
- gastroparesis
- gastropathy
- gastrula
- gastrulation
- gaststätte
- gasworks
- gat
- gata
- gata-
- gata-binding
- gataaa
- gataaccttcttggcaccag
- gatas
- gataway
- gatcctcgagatcgataccgtcgagctagcgtagtctgggacgtcgtatgggtatcgactcatattcgagatatttgattataccaaggc-
- gatcctcgagttacattggttttatatctggg
- gatcggtaccattagcacggtgccttaacca-
- gatch
- gate
- gatekeeper
- gatekeepers
- gb
- gc
- gdna
- gdpb
- ggg
Example sentences for: gat
How can you use “gat” in a sentence? Here are some example sentences to help you improve your vocabulary:
A cDNA that encodes a hemagglutinin-tagged NPM3 protein was produced by PCR under the following conditions: 100 μl reactions with 1X Pfu buffer, 0.2 mM deoxyribonucleotides, 1 μM HA-F primer (5'-AAA GAA TTC AGC ATG TAC CCA TAC GAC GTC CCA GAC TAC GCC GCC GCC GGT ACT GCA GCT GCC-3'), 1 μM NPM3 -R2 primer (5'-AAA GAA TTC CTA GGG CCT GCC CCC CTG CTT TTT GGC AGG AAG GAT GGG-3'), 1 ng of human NPM3 cDNA insert, and 2.5 Units of recombinant Pfu DNA Polymerase.
The introns were PCR-amplified with KM37 (GAT AAT ACG ACT CAC TAT AAT GGC ATT ACC GCC TTG T) and GM24 (GCT CTA GAC TTA GCT ACA ATA TGA AC) in 25 μl reactions under the conditions stated above and cycled 20 times.
Binding reactions were carried out at room temperature for 30 min in a total volume of 15 μl and contained 2-5 μg of nuclear extracts, 5 μl of 5X gel shift binding buffer (20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris-HCl, pH 7.5), 2 μg poly (dI-dC) and 3 × 10 4cpm/μl of [ 32P] labeled CREB (5'-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3'), NF-κB (5'-AGT TGA GGG GAC TTT CCC AGG C-3') (Promega Gel Shift Assay Systems, Madison, WI, USA) or CCAAT/enhancer binding protein (C/EBP) (5'-TGC AGA TTG CGC AAT CTG CA-3') (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) oligonucleotides.
2: CTA G AG GGG AAT TGT TAT CCG CTC ACA ATT CCC CTA TAG TGA GTG GTA TTA ATT TCG CGG GAT CGA (XbaI site in bold)
1 M), d(GAT)TP (25 mM), dCTP (1 mM), Cy3-dCTP (2 mM)(Amersham, Piscataway, NJ) and Superscript RT II (200 units) were added and incubated at room temperature for 10 minutes and then at 42°C for 2 hours.
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