Example sentences for: cdna

How can you use “cdna” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • All of these skin fibroblast samples had previously been screened with a cDNA amplification/SSCA protocol [ 14 ] and a genomic DNA/exon amplification method [ 15 ] with negative results.

  • Preparation and sequencing of cDNA

  • Primers used for the amplification of the total NF1 cDNA in 5 overlapping fragments were as described [ 17 ] . Reactions were performed as described [ 13 ] . The PCR products were cloned in the pCR2.

  • The final assemblies of EST and cDNA or mRNA sequences were aligned to the index NT_017021 genomic sequence using the Martinez Needleman-Wunsch algorithm in MegAlign (DNA Star, Lasergene) permitting identification of exon-intron boundaries, new exons (P24), and the direction of transcription relative to the telomere.

  • Detailed procedures for preparation of cDNA and fluorescently labeled cRNA, hybridization, staining, and scanning of gene chips and assay monitoring are outlined in earlier literature [ 15 ] . The platform chosen for global expression profile was the Rat Genome U34 Array (Affymetrix, Santa Clara, California), which contains probes for a total of 8799 expressed sequence tag (EST) clusters and genes (including controls).


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