Words similar to cdna-based
Example sentences for: cdna-based
How can you use “cdna-based” in a sentence? Here are some example sentences to help you improve your vocabulary:
As mentioned before, the N-encapsidated genome-length RNAs are indeed extremely resistant to nucleases, to the extent that formation of such nuclease-resistant RNA products is in fact considered a defining criterion for viral replication in vitro and in vivo [ 32 ] . While the resistance of the viral genomic RNA to dsRNA has important ramifications for antiviral therapy, it is also clear that the dsRNA approach cannot be used against cis -acting NNR viral genomic sequences such as the intergenic regions, for which the cDNA-based approach will continue to be the method of choice [ 16 ] . It is obvious that a creative combination of the two techniques will lead to exciting possibilities in the reverse genetics of RNA viruses and in the antiviral regimen.
In this patient, the DNA-based assay provided examples of clonal expansions that were present in only one joint (eg the V H 3-J H and V H 5-J H combinations in Figure 4), and the companion cDNA-based assays indicated that in some instances these expansions were either activated or resting.
Conversely, in the setting of specific B-cell clonal activation without concomitant clonal expansion, the cDNA-based assay will indicate a dominant HCDR3 length, whereas the DNA-based assay may not.
Our results thus offer a mechanism of specific and direct ablation of RNA-based gene expression and a quicker and simpler alternative to cDNA-based reverse genetics of RNA viruses.
Since our laboratory is interested in deciphering the temporal signaling pathways in host-RSV interaction and the role of RSV gene products in the process, we have sought potential alternatives to the cDNA-based approach that might allow us to study the effect of functional loss of a specific RSV gene product during the course of a standard virus infection in cell culture.
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