Words similar to cdna-based
Example sentences for: cdna-based
How can you use “cdna-based” in a sentence? Here are some example sentences to help you improve your vocabulary:
Despite its revolutionary effect on RNA viral reverse genetics, however, the cDNA-based strategy is not without limitations.
These obstacles have been largely circumvented by the use of cloned viral cDNA that is then altered by standard DNA-based site-directed mutagenesis procedures [ 8 9 ] . Originally designed for influenza virus minigenomes [ 10 ] , such cDNA-based "reverse genetics" strategy has been adopted in a large number of NNR viruses, including vesicular stomatitis virus (VSV), respiratory syncytial virus (RSV), and measles, to name a few [ 11 12 13 14 ] . Recently, extension of this approach has resulted in the cloning of full-length viral cDNA capable of producing infectious recombinant virus particles upon transcription.
Conversely, in the setting of specific B-cell clonal activation without concomitant clonal expansion, the cDNA-based assay will indicate a dominant HCDR3 length, whereas the DNA-based assay may not.
In order to confirm that these clonally expanded B cells were receiving ongoing antigenic stimulation and not limited solely to the memory compartment, we employed the cDNA-based assay to distinguish clonal expansions of activated B cells from resting (memory) cells.
In this patient, the DNA-based assay provided examples of clonal expansions that were present in only one joint (eg the V H 3-J H and V H 5-J H combinations in Figure 4), and the companion cDNA-based assays indicated that in some instances these expansions were either activated or resting.