Words similar to cdna-based
Example sentences for: cdna-based
How can you use “cdna-based” in a sentence? Here are some example sentences to help you improve your vocabulary:
These obstacles have been largely circumvented by the use of cloned viral cDNA that is then altered by standard DNA-based site-directed mutagenesis procedures [ 8 9 ] . Originally designed for influenza virus minigenomes [ 10 ] , such cDNA-based "reverse genetics" strategy has been adopted in a large number of NNR viruses, including vesicular stomatitis virus (VSV), respiratory syncytial virus (RSV), and measles, to name a few [ 11 12 13 14 ] . Recently, extension of this approach has resulted in the cloning of full-length viral cDNA capable of producing infectious recombinant virus particles upon transcription.
Despite its revolutionary effect on RNA viral reverse genetics, however, the cDNA-based strategy is not without limitations.
Our results thus offer a mechanism of specific and direct ablation of RNA-based gene expression and a quicker and simpler alternative to cDNA-based reverse genetics of RNA viruses.
Since our laboratory is interested in deciphering the temporal signaling pathways in host-RSV interaction and the role of RSV gene products in the process, we have sought potential alternatives to the cDNA-based approach that might allow us to study the effect of functional loss of a specific RSV gene product during the course of a standard virus infection in cell culture.
In order to confirm that these clonally expanded B cells were receiving ongoing antigenic stimulation and not limited solely to the memory compartment, we employed the cDNA-based assay to distinguish clonal expansions of activated B cells from resting (memory) cells.
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