Words similar to cdna-based
Example sentences for: cdna-based
How can you use “cdna-based” in a sentence? Here are some example sentences to help you improve your vocabulary:
We analyzed the peripheral blood, synovial fluid, and synovial tissue B cells of rheumatoid arthritis patients ( n = 20, 10, and 5, respectively) using the genomic DNA- and cDNA-based fingerprinting assays to develop an understanding of the diversity of the B cells in these compartments.
Despite its revolutionary effect on RNA viral reverse genetics, however, the cDNA-based strategy is not without limitations.
These obstacles have been largely circumvented by the use of cloned viral cDNA that is then altered by standard DNA-based site-directed mutagenesis procedures [ 8 9 ] . Originally designed for influenza virus minigenomes [ 10 ] , such cDNA-based "reverse genetics" strategy has been adopted in a large number of NNR viruses, including vesicular stomatitis virus (VSV), respiratory syncytial virus (RSV), and measles, to name a few [ 11 12 13 14 ] . Recently, extension of this approach has resulted in the cloning of full-length viral cDNA capable of producing infectious recombinant virus particles upon transcription.
Our results thus offer a mechanism of specific and direct ablation of RNA-based gene expression and a quicker and simpler alternative to cDNA-based reverse genetics of RNA viruses.
As mentioned before, the N-encapsidated genome-length RNAs are indeed extremely resistant to nucleases, to the extent that formation of such nuclease-resistant RNA products is in fact considered a defining criterion for viral replication in vitro and in vivo [ 32 ] . While the resistance of the viral genomic RNA to dsRNA has important ramifications for antiviral therapy, it is also clear that the dsRNA approach cannot be used against cis -acting NNR viral genomic sequences such as the intergenic regions, for which the cDNA-based approach will continue to be the method of choice [ 16 ] . It is obvious that a creative combination of the two techniques will lead to exciting possibilities in the reverse genetics of RNA viruses and in the antiviral regimen.
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