Example sentences for: cdp-star

How can you use “cdp-star” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Samples of known numbers of cells from the cell lines and recombinant, purified Tag prepared from a baculovirus lysate [ 40 ] were loaded on 10% polyacrylamide discontinuous mini-gels (Bio-Rad, Hercules, CA) and electrophoresed conventionally [ 41 ] . Gels were then electrophoretically blotted [ 42 ] onto PVDF membrane (Bio-Rad) without methanol for 15 min at 100 V. Tag was visualized by immunostaining with PAb416 and alkaline phosphatase conjugated secondary antibody (Sigma) using chemiluminescent detection with CDP-Star substrate according to the manufacturers directions (Tropix, Bedford, MA).

  • Incubation was then performed for ten minutes at room temperature with CDP-Star chemiluminescent substrate (Boehringer Mannheim, Biochemical Products, Indianapolis IN) diluted 1:100 in substrate buffer.


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