Words similar to cdm-fe
Example sentences for: cdm-fe
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The iron uptake by A. baumannii ATCC 19606 when grown in CDM-Fe was inhibited more than 90% by monoclonal antibodies directed against IROMPs of A. bauamannii, as compared to bacteria grown in CDM+Fe (Fig.
At the end the unbound radiolabelled iron was removed by chromatography using sephadex G-10 as described by Rosenberg [ 44 ] . The uptake of radiolabelled iron siderophore was studied by the method described by Rosenberg [ 44 ] . Briefly A. baumannii was grown in CDM-Fe medium also in CDM+Fe medium to a mid exponential phase and was washed twice with uptake medium (30 mM Na 2 HPO 4 , 10 mM K 2 HPO 4 , 10 mM (NH 4 ) 2 SO 4 ,1 mM MgCl 2 and 40 μM CaCl 2 pH 6.9) at 4°C and re-suspended in the same medium at a density of A 470 = 0.4.
The uptake of radiolabeled iron siderophore complex by the bacterial whole cells in CDM-Fe was significantly more than that in case of bacterial cells obtained from CDM+Fe medium (Fig.
A. baumannii ATCC 19606 (10 7bacterial cells/100 μl) grown in CDM-Fe medium were incubated with 100 μl of monoclonal antibody culture supernatant for 30 min at 37°C.
This strongly indicates that OMP of CDM-Fe grown bacteria contains the IROMPs and that they bind to the iron siderophore complex, hence they may be the receptor for siderophore iron complex.