Words similar to cdm-fe

Example sentences for: cdm-fe

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For studying the role of monoclonal antibodies in blocking of iron uptake pathway, we purified the siderophore by a modified method of Smith [ 17 ] . Briefly the culture supernatant of bacteria grown in CDM-Fe medium was extracted twice with ethyl acetate.
At the end the unbound radiolabelled iron was removed by chromatography using sephadex G-10 as described by Rosenberg [ 44 ] . The uptake of radiolabelled iron siderophore was studied by the method described by Rosenberg [ 44 ] . Briefly A. baumannii was grown in CDM-Fe medium also in CDM+Fe medium to a mid exponential phase and was washed twice with uptake medium (30 mM Na 2 HPO 4 , 10 mM K 2 HPO 4 , 10 mM (NH 4 ) 2 SO 4 ,1 mM MgCl 2 and 40 μM CaCl 2 pH 6.9) at 4°C and re-suspended in the same medium at a density of A 470 = 0.4.
The iron uptake by A. baumannii ATCC 19606 when grown in CDM-Fe was inhibited more than 90% by monoclonal antibodies directed against IROMPs of A. bauamannii, as compared to bacteria grown in CDM+Fe (Fig.
(A) OMPs expressed by bacteria grown in CDM-Fe medium i.e. antigen A
It was found that all the five monoclonal antibodies are bactericidal and they specifically kill the bacteria grown in CDM-Fe media.