Words similar to cdm-fe

Example sentences for: cdm-fe

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For studying the role of monoclonal antibodies in blocking of iron uptake pathway, we purified the siderophore by a modified method of Smith [ 17 ] . Briefly the culture supernatant of bacteria grown in CDM-Fe medium was extracted twice with ethyl acetate.
At the end the unbound radiolabelled iron was removed by chromatography using sephadex G-10 as described by Rosenberg [ 44 ] . The uptake of radiolabelled iron siderophore was studied by the method described by Rosenberg [ 44 ] . Briefly A. baumannii was grown in CDM-Fe medium also in CDM+Fe medium to a mid exponential phase and was washed twice with uptake medium (30 mM Na 2 HPO 4 , 10 mM K 2 HPO 4 , 10 mM (NH 4 ) 2 SO 4 ,1 mM MgCl 2 and 40 μM CaCl 2 pH 6.9) at 4°C and re-suspended in the same medium at a density of A 470 = 0.4.
The iron uptake by A. baumannii ATCC 19606 when grown in CDM-Fe was inhibited more than 90% by monoclonal antibodies directed against IROMPs of A. bauamannii, as compared to bacteria grown in CDM+Fe (Fig.
This strongly indicates that OMP of CDM-Fe grown bacteria contains the IROMPs and that they bind to the iron siderophore complex, hence they may be the receptor for siderophore iron complex.
It was found that all the five monoclonal antibodies are bactericidal and they specifically kill the bacteria grown in CDM-Fe media.