Words similar to cdk
Example sentences for: cdk
How can you use “cdk” in a sentence? Here are some example sentences to help you improve your vocabulary:
In the present study we show that RA, by inhibiting cyclin H and cdk7 expression, reduces phosphorylation of RARα.
Studies in budding yeast identified SCF Cdc4p, an E3 ubiquitin ligase complex that mediates the ubiquitylation of the CDK inhibitor Sic1p [ 3 4 ] . SCF Cdc4pconsists of at least four proteins: the cullin Cdc53p, the RING domain protein Hrt1p/Rbx1p/Roc1p, the adapter protein Skp1p, and Cdc4p (reviewed in [ 1 ] ). Cdc4p contains two sequence motifs, which are conserved in a wide variety of so-called F-box proteins: C-terminal WD-repeats that are involved in binding the substrate Sic1p in a phosphorylation-dependent manner, and a central F-box [ 5 ] that interacts with Skp1p [ 6 7 ] . Cdc53p in turn binds to Skp1p and Hrt1p/Rbx1p/Roc1p, which bridges Cdc53p with the ubiquitin-conjugating enzyme Cdc34p/Ubc3p [ 6 8 9 10 11 12 ] . In vitro reconstitution demonstrated that SCF Cdc4p, Cdc34p, E1, ubiquitin, and ATP are sufficient to mediate Sic1p polyubiquitylation [ 6 7 ] .
In parallel, the expression of cyclin A, an essential component of certain cyclin-dependent kinases and absolutely required for the progression of cells through the cell cycle [ 38 39 ] , was down-regulated under these conditions (Figure 1), consistent with earlier observations [ 27 28 31 ] . Furthermore, expression of the CDK inhibitor p27 Kip1was strongly increased in suspension cells (Figure 1), similar to what has been observed in other cell types after the disruption of cell-matrix interactions [ 29 31 32 ] . Finally, as we have reported before [ 31 ] , the expression of cdk4, one of the catalytic subunits of CDKs, was not significantly altered in suspension culture cells and therefore could be used as a loading control for the Western blot analysis.
We determined whether the inhibition of transcription is specific to flavopiridol by comparing gene expression profiles of flavopiridol-treated cells with those of cells treated with the CDK inhibitors roscovitine and 9-nitropaullone.
1A, RA treatment decreased expression of both cyclin H and cdk7 in SCC4 and SCC25 cells by 3 fold.
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