Words similar to cdc
Example sentences for: cdc
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, manuscript in preparation), but net1 tab 2-1bypassed cdc5Δ with very low efficiency, presumably because CDC5 has other essential functions [ 44 25 26 45 ] .
To assemble Net1N beads with Cdc14 for six reactions (Figure 4A, detection method: Coomassie Blue staining), 105 μl of anti-T7 beads (Novagen) were incubated with 24 μg of His6-T7-Net1N in 100 μl of HEBD for 1 hr, washed four times with HBS + 0.2% n-octylglucoside + 2 mM DTT, and incubated with 6 μg of GST-Cdc14 in 100 μl of HEBD for 1 hr.
Mtr10 assembles with and functions as a nuclear import receptor for the mRNA-binding protein Npl3 [ 36 37 ] . Interestingly, srp1-31 also inhibits nuclear import of multiple proteins including Npl3 [ 51 52 ] . Remarkably, reduction-of-function alleles of the karyopherin KAP104 were also recovered in a screen for mutations that suppressed cdc15-2 ts (A.
This small member of the Ras GTPase superfamily has been implicated in fibroblast models of integrin dependent actin stress fiber formation, focal contact assembly [ 13 ] , and gene induction via the serum response element [ 14 ] . Studies of Rho function have been facilitated by the use of specific toxins such as Clostridium botulinum toxin C3 which catalyses the ADP-ribosylation and inactivation of Rho [ 15 16 17 ] . The function of this GTPase and related family members such as Rac and Cdc42 has been extensively studied in the fibroblast model.
The release of Cdc14 from nucleolar Net1 promotes mitotic exit [ 15 17 18 ] . net1 tab 2-1cells display a panel of nucleolar defects including reduction of nucleolar silencing, mislocalization of nucleolar antigens, alteration of rDNA morphology, and reduction of rRNA synthesis [ 16 18 ] . To test whether perturbation of nucleolar structure is consistently associated with bypass of cdc15Δ, we immunolocalized the nucleolar antigen Nop1 in all tab mutants.
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