Example sentences for: biotinylated

How can you use “biotinylated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Primer NV-2 was biotinylated to facilitate binding of the product to a streptavidin (Sigma-Aldrich) -coated microtiter plate (Immulon II Thermo Labsystems, Franklin, MA, USA).

  • After washing twice, cells were incubated with 1 or 2 μg/10 6cells in 100 μl biotinylated goat anti-human IL-6 receptor (ligand-affinity purified; R&D Systems) on ice for 45 min.

  • Poly(A) +cDNA was transcribed in vitro by incorporation of biotinylated CTP and UTP (Enzo Diagnostics, Farmingdale, NY) using a BioArray High Yield RNA Transcript Labeling kit according to the manufacturer's instructions.

  • Approximately 200 ng of the pre-amplified AFLP fragments (length range from 100 bp - 600 bp) were added to a single reaction mixture containing 4.2 × SSC (Saline-Sodium Citrate, pH 7.0), 0.07% SDS (sodium dodecyl sulfate), and 10 pmol biotinylated probe.

  • The second round of IVT of cRNA was as in the first round but with biotinylated ribonucleotides rather than non-biotinylated ribonucleotides.


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