Words similar to biotin-labeled
Example sentences for: biotin-labeled
How can you use “biotin-labeled” in a sentence? Here are some example sentences to help you improve your vocabulary:
Metaphase spreads hybridized with biotin-labeled HPV-16 probe detected with biotinyl-tyramide and streptavidin-FITC, consistently demonstrated intense signal in the central region of the long arm of an acrocentric chromosome (Figures 1Eand 1F).
Plasmid/insert DNA sequences were isolated from bacterial cultures by column purification (Qiagen, Valencia, CA) and were biotin-labeled by nick-translation (Invitrogen, Carlsbad, CA).
Total RNA (20 μg) was used to synthesize double-stranded cDNA using Superscript ®Choice System (Life Technologies), and the template for an in vitro transcription reaction was used to synthesize biotin-labeled antisense cRNA (BioArray™ High Yield RNA Transcript Labeling Kit; Enzo Diagnostics, Farmingdale, NY, USA).
To quantitate differences in activated (phosphorylated) p53 protein, 3 SN50 and control E15 + 2 explants were sectioned, preincubated with unlabeled goat-anti mouse IgG as previously described [ 9 ] and sequentially incubated with a monoclonal anti-phosphorylated p53 (Ser15) antibody (Cell Signaling Technology, Beverly, MA), biotin-labeled goat anti-mouse IgG, and HRP-labeled SA (Zymed Laboratories, South San Francisco, CA), and counterstained with hematoxylin.
While screening the SSR-enriched, small-insert libraries can significantly reduce time and cost [ 11 ] . Enrichment can be realized by hybridizing SSR-containing fragments with biotin-labeled probes that are either captured by magnetic beads coated with streptavidin or fixed on a nitrate filter [ 12 ] . The eluted portion after removing non-hybridized DNA was highly enriched for microsatellites, with enrichment efficiency between 50-90% [ 13 ] .
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