Words similar to biotin-conjugated

Example sentences for: biotin-conjugated

How can you use “biotin-conjugated” in a sentence? Here are some example sentences to help you improve your vocabulary:

Splenocytes or lymph node cells were stained for flow cytometry with the PE- or biotin-conjugated monoclonal antibodies indicated in the appropriate figure legends as previously described [ 28 ] . Cells stained with biotinylated primary antibody were detected with streptavidin-Cy-Chrome (Pharmingen).
Embryos were blocked with BBT/NGS for 30 minutes prior to incubation with the biotin-conjugated secondary antibodies in BBT/NGS for at least 2 hours at room temperature or overnight at 4°C.
After washing, bound antibody isotope was detected with biotin-conjugated antimouse whole IgG (heavy and light chain) antibody (Pharmingen, San Diego, CA, USA).
Cytoplasmic IFNgR1 and IFNgR2 receptor subunits were detected by using a procedure described for intracellular cytokine staining [ 50 ] . Briefly, cells were fixed in ice-cold 4% formaldehyde for 5 min, washed 2 times, permeabilized in staining buffer containing 0.2% saponin for 60 min at 4°C, incubated with biotin-conjugated mAb's (specific or isotype-matched) for 30 min, washed 2 times, incubated with SA-PE and washed again (saponin was present in staining buffer at all times).
Staining for cell surface IFNg receptors was performed similarly, except that cells were first incubated with biotin-conjugated mAb's (specific or isotype-matched) for 30 min, washed 2 times and then stained with SA-Alf488.