Words similar to biotin
Example sentences for: biotin
How can you use “biotin” in a sentence? Here are some example sentences to help you improve your vocabulary:
This design resulted in a uniform array of biotinylated tiles, with each biotin moiety separated by about 19 nm.
Visual examination of the structure of this insert domain revealed a topology with elements of both a sandwich and a barrel, and two "waist"-like structures that are characteristic of the sandwich-barrel hybrid motif (SBHM) fold [ 75 76 ] (see also the SCOP database [ 65 ] ). The SBHM fold is present in biotin/lipoate carrier domains and their homologs found in a variety of enzymes and transporters [ 76 ] . A DALI search with this insert domain from the β subunit of Thermus DDRP showed similarity to the SBHMs from various biotin/lipoate-binding enzymes, such as acetyl-CoA carboxylase (1bdo) or dihydrolipoamide acetyltransferase (1iyu) with Z scores of ~6 and RMSD ~2.
05% Tween 20 and biotin labeled anti-mouse IgG (100 μl/well; 125 ng/ml diluted in PBS containing 1% dry fat-free milk) was added.
Neutralite avidin (Southern Biotechnology Associates) (25 μg in 50 μl PBST per spot) was added to the spot areas within plastic rings, which had been treated with sulfo-NHS-LC-biotin, and allowed to bind to biotin moieties on the well surface.
The cDNA was purified using a Qiagen QIAquick purification kit, dried down, and resuspended in IVT reaction mix, containing 3.0 μl nuclease-free water, 4.0 μl 10X reaction buffer, 4.0 μl 75 mM ATP, 4.0 μl 75 mM GTP 3.0 μl 75 mM CTP, 3.0 μl 75 mM UTP, 7.5 μl 10 mM Biotin-11-CTP, 7.5 μl 10 mM Biotin 11-UTP and 4.0 μl enzyme mix (unmodified control condition).
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