Words similar to biotin
Example sentences for: biotin
How can you use “biotin” in a sentence? Here are some example sentences to help you improve your vocabulary:
The digestion step was repeated twice, and the 3 cell isolates were pooled in mouse osteoblast growth medium (OBGM), consisting of Dulbecco's modified Eagle's medium containing 25 mM HEPES, 10% fetal bovine serum (Sigma), 2 g of sodium bicarbonate per liter, 75 μg of glycine/ml, 100 μg of ascorbic acid/ml, 40 ng of vitamin B 12 /ml, 2 μg of p -aminobenzoic acid/ml, 200 ng of biotin/ml, and penicillin (100 U/ml)-streptomycin (100 μg/ml)-amphotericin B (25 μg/ml) (pH 7.4) [ 60 ] . Cells were seeded in six-well cluster plates and incubated at 37°C in a 5% CO 2 atmosphere until they reached confluence (6 to 7 days).
First, spots of biotin moieties on the wells were created by using sulfo-NHS-LC-biotin.
To the biotin moieties on the solid surface, biotinylated adenoviral vectors were attached by using avidin as a molecular bridge between the biotin moieties on the solid surface and the viral surface.
10 TCR) were purified from ascites and conjugated with fluorescein isothiocyanate (FITC), biotin or phycoerythrin (PE) by Dr. Roger Lallone (Brookwood Biomedical; Birmingham, AL).
It has been noticed that if a patient is suspected of having a mitochondrial disease, abnormal carnitine esters may help with the diagnosis, [ 38 ] and abnormal acylcarnitine profiles do not exclusively indicate fatty acid oxidation disorders [ 39 ] . Although the abnormal carnitine ester present in the proband is elevated in organic acidemias and is not usually present in mitochondrial cytopathies, we speculate that due to compromised ATP synthesis, secondary alterations may be generated in these patients by changes in mitochondrial protein synthesis, decrease in Krebs cycle intermediates and increased free radical formation, that could be responsible for a secondary impairment of mitochondrial biotin dependent carboxylases, but further elucidation is necessary before we could arrive to a conclusion in this regard.
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