Example sentences for: biotin

How can you use “biotin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 05% Tween 20, and biotin labeled anti-mouse IgG (100 μl/well; 125 ng/ml diluted in PBS containing 1% dry fat-free milk) was added.

  • A PSI-BLAST search started with the N-terminal SBHM from the Aquifex aeolicus DDRP β' subunit detected several SBHMs from proteins other than the β' subunit, such as acetylornithine deacetylase (E = 2 × 10 -7, iteration 2), the C-terminal region of bacterial cytochrome F (E = 10 -4, iteration 2), biotin carboxyl carrier domain of biotin transcarboxylases (E = 6 × 10 -4, iteration 2) and the phosphotransferase system enzyme II (E = 10 -3, iteration 2).

  • Of these proteins, GRP 94, GRP 78, Calreticulin and HSP 90 can be surface-labeled by biotin and appear to be localizing to the egg surface based on preliminary immunofluoresence data.

  • The wide spread in different phylogenetic lineages of two other SBHM domains, namely those found in dihydrolipoamide acetyltransferase and the glycine-cleaving enzyme, is indicative of their presence in LUCA [ 76 ] . The SBHM of the DDRPs is specifically related to the biotin/lipoate-binding SBHMs.

  • They showed that Alexa 488 was brighter and more stable than fluorescein when conjugated to biotin binding proteins, goat anti-mouse IgG and its F(ab')2 fragments.


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