Words similar to biotin
Example sentences for: biotin
How can you use “biotin” in a sentence? Here are some example sentences to help you improve your vocabulary:
single strand) probe fragments was checked by agarose gel electrophoresis of denatured probe followed by Southern transfer and demonstration of biotin by BluGene kit (Invitrogen Life Technologies).
After the removal of unbound Neutralite avidin, Ad5.CMV-LacZ, which had been biotinylated by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was applied to the wells and allowed to bind to unoccupied biotin-binding sites of Neutralite avidin that had been bound to biotin moieties on the solid surface.
This procedure is explained in detail in Coonrod et al, 1999 [ 8 ] . Briefly, after collection zona-free eggs were washed six times in BWW medium (Irvine Scientific, Santa Ana, CA) containing 100 μg/ml polyvinylalcohol (PVA, Sigma, St. Louis, MO), biotinylated with 2 mg/ml Sulfo-NHS biotin (Pierce, Rockford, IL) in BWW/PVA for 7 min at room temperature, and washed six times in BWW/PVA.
A total of 8 μg each of H9 (control sample) and H9/Tat (test sample) mRNAs were reverse transcribed into Biotin and Dinitrophenyl (DNP) labeled cDNA, respectively.
Specific transduction of target cells is seen only at the locations (spots) where biotin moieties were initially attached.