Words similar to biotin
Example sentences for: biotin
How can you use “biotin” in a sentence? Here are some example sentences to help you improve your vocabulary:
BAC and PAC DNAs, isolated from bacterial cultures (Nucleobond, Clontech, Franklin Lakes, NJ), were labeled by nick-translation with biotin and digoxigenin.
Frozen sections (8 μm) of the immediately snap-frozen tissue sample were screened for B and T cells by double immunohistological staining with anti-CD20 and anti-CD3 monoclonal antibodies using the alkaline phosphatase-anti-alkaline phosphatase and streptavidin biotin horseradish peroxidase technique [ 17, 18].
The cDNA was purified using a Qiagen QIAquick purification kit, dried down, and resuspended in IVT reaction mix, containing 3.0 μl nuclease-free water, 4.0 μl 10X reaction buffer, 4.0 μl 75 mM ATP, 4.0 μl 75 mM GTP 3.0 μl 75 mM CTP, 3.0 μl 75 mM UTP, 7.5 μl 10 mM Biotin-11-CTP, 7.5 μl 10 mM Biotin 11-UTP and 4.0 μl enzyme mix (unmodified control condition).
One of the amplification primers includes a 5' biotin tag, which is targeted in a streptavidin-magnetic bead purification step to generate a single stranded template for the primer extension reaction.
Visual examination of the structure of this insert domain revealed a topology with elements of both a sandwich and a barrel, and two "waist"-like structures that are characteristic of the sandwich-barrel hybrid motif (SBHM) fold [ 75 76 ] (see also the SCOP database [ 65 ] ). The SBHM fold is present in biotin/lipoate carrier domains and their homologs found in a variety of enzymes and transporters [ 76 ] . A DALI search with this insert domain from the β subunit of Thermus DDRP showed similarity to the SBHMs from various biotin/lipoate-binding enzymes, such as acetyl-CoA carboxylase (1bdo) or dihydrolipoamide acetyltransferase (1iyu) with Z scores of ~6 and RMSD ~2.
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