Words similar to biotinyl-s
Example sentences for: biotinyl-s
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After 4 h at 20°C, the wells were washed and bound gp120 was detected by addition of biotinyl-sCD4 (1 μg) followed by HRP-streptavidin as described above.
1insert), corresponding to a molar ratio of ≥ 1.2 [ 32 ] , in agreement with the observation that gp120-CD4 complexes consist of one molecule each of CD4 and gp120 [ 16 ] . Biotinyl-sCD4 in the absence of gp120 did not bind to CAP.
To measure the binding of biotinyl-sCD4 and biotinyl-gp120 (in the presence and absence of sCD4), respectively, to CAP, the biotinylated proteins were added to CAP and control postcoated wells without CAP, respectively, at dilutions shown in Fig.