Words similar to biotinyl-s
Example sentences for: biotinyl-s
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To measure the binding of biotinyl-sCD4 and biotinyl-gp120 (in the presence and absence of sCD4), respectively, to CAP, the biotinylated proteins were added to CAP and control postcoated wells without CAP, respectively, at dilutions shown in Fig.
After 4 h at 20°C, the wells were washed and bound gp120 was detected by addition of biotinyl-sCD4 (1 μg) followed by HRP-streptavidin as described above.
1insert), corresponding to a molar ratio of ≥ 1.2 [ 32 ] , in agreement with the observation that gp120-CD4 complexes consist of one molecule each of CD4 and gp120 [ 16 ] . Biotinyl-sCD4 in the absence of gp120 did not bind to CAP.