Words similar to biotinylation
Example sentences for: biotinylation
How can you use “biotinylation” in a sentence? Here are some example sentences to help you improve your vocabulary:
These spot areas within plastic rings were treated with 1 mg/ml sulfo-NHS-LC-biotin in PBS (pH 7.4) (50 μl per spot) for 2 h at 4°C and then washed with PBST to remove unreacted biotinylation reagent.
Portable Network Graphic (PNG) File showing enhanced cytoskeletal immobilization and tyrosine phosphorylation of cellular proteins in response to stimulation with mature TGFβ 1 . Cytoskeletally anchored α V β 6 was immunoprecipitated after TGFβ 1 stimulation (10 nM for 10 minutes) followed by Western analysis with antibodies against tyrosine-phosphorylated proteins (A) or Western blotting after biotinylation of all proteins and streptavidin detection (B).
During the biotinylation reaction, plastic rings (inner diameter, 1.4 cm) and agarose were used to delimit the regions of biotinylation on the solid surface and to protect the remaining areas from biotinylation, respectively.
Biotinylation and avidin blotting identifies surface-labeled egg proteins
After unreacted biotinylation reagent was removed, excess Neutralite avidin (a deglycosylated, neutralized form of avidin; Southern Biotechnology Associates, Alabama, AL) was added, which should bind to biotin moieties on the solid surface.