Words similar to biotinylation
Example sentences for: biotinylation
How can you use “biotinylation” in a sentence? Here are some example sentences to help you improve your vocabulary:
Ad5.CMV-LacZ was treated with varying concentrations (0 - 30 μg/ml) of sulfo-NHS-LC-biotin, and non-virion-associated biotinylation reagent was removed by repeated ultrafiltration.
These results suggest that, upon biotinylation of the viral surface under controlled conditions, adenoviral vectors can be immobilized on a solid surface by using the (strept)avidin-biotin interaction, and that such immobilized adenoviral vectors are capable of infecting cells, in situ , on the solid surface.
Very limited numbers of infected cells were present outside the biotinylation areas, indicating that biotinylated Ad5.CMV-LacZ was bound specifically to biotin moieties on the well surface via Neutralite avidin.
Portable Network Graphic (PNG) File showing enhanced cytoskeletal immobilization and tyrosine phosphorylation of cellular proteins in response to stimulation with mature TGFβ 1 . Cytoskeletally anchored α V β 6 was immunoprecipitated after TGFβ 1 stimulation (10 nM for 10 minutes) followed by Western analysis with antibodies against tyrosine-phosphorylated proteins (A) or Western blotting after biotinylation of all proteins and streptavidin detection (B).
During the biotinylation reaction, plastic rings (inner diameter, 1.4 cm) and agarose were used to delimit the regions of biotinylation on the solid surface and to protect the remaining areas from biotinylation, respectively.