Words similar to biotinylation
Example sentences for: biotinylation
How can you use “biotinylation” in a sentence? Here are some example sentences to help you improve your vocabulary:
Portable Network Graphic (PNG) File showing enhanced cytoskeletal immobilization and tyrosine phosphorylation of cellular proteins in response to stimulation with mature TGFβ 1 . Cytoskeletally anchored α V β 6 was immunoprecipitated after TGFβ 1 stimulation (10 nM for 10 minutes) followed by Western analysis with antibodies against tyrosine-phosphorylated proteins (A) or Western blotting after biotinylation of all proteins and streptavidin detection (B).
These spot areas within plastic rings were treated with 1 mg/ml sulfo-NHS-LC-biotin in PBS (pH 7.4) (50 μl per spot) for 2 h at 4°C and then washed with PBST to remove unreacted biotinylation reagent.
Biotinylation of adenoviral vectors with sulfo-NHS-LC-biotin
We previously showed that biotin moieties can be attached covalently to the outer surface of adenoviral vectors without appreciable effect on their infectivity under carefully controlled biotinylation conditions [ 10 11 ] . Such biotinylated viral particles could be attached, specifically and stably, to a solid surface on which (strept)avidin is covalently immobilized (avidin- or streptavidin-coated solid surface).
Ad5.CMV-LacZ was treated with varying concentrations (0 - 30 μg/ml) of sulfo-NHS-LC-biotin, and non-virion-associated biotinylation reagent was removed by repeated ultrafiltration.
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