Words similar to biotinylation
Example sentences for: biotinylation
How can you use “biotinylation” in a sentence? Here are some example sentences to help you improve your vocabulary:
Very limited numbers of infected cells were present outside the biotinylation areas, indicating that biotinylated Ad5.CMV-LacZ was bound specifically to biotin moieties on the well surface via Neutralite avidin.
Plastic rings (inner diameter, 1.4 cm) were used to specify the biotinylation areas, and the remaining area of the wells was covered with a layer of 2% agarose in PBS.
This result is in contrast with our previous study, which showed that greater than 95% of biotinylated Ad5.CMV-LacZ, prepared under the same conditions, can be readily captured on streptavidin-coated microbeads [ 10 ] . Thus, the relatively low immobilization efficiency of biotinylated Ad5.CMV-LacZ on the surface of streptavidin-coated wells is not attributable to the absence of accessible biotin moieties on the surfaces of a fraction of viral particles or the presence of unreacted biotinylation reagent in the biotinylated Ad5.CMV-LacZ preparations.
These spot areas within plastic rings were treated with 1 mg/ml sulfo-NHS-LC-biotin in PBS (pH 7.4) (50 μl per spot) for 2 h at 4°C and then washed with PBST to remove unreacted biotinylation reagent.
We previously showed that biotin moieties can be attached covalently to the outer surface of adenoviral vectors without appreciable effect on their infectivity under carefully controlled biotinylation conditions [ 10 11 ] . Such biotinylated viral particles could be attached, specifically and stably, to a solid surface on which (strept)avidin is covalently immobilized (avidin- or streptavidin-coated solid surface).
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