Words similar to biotinylation

Example sentences for: biotinylation

How can you use “biotinylation” in a sentence? Here are some example sentences to help you improve your vocabulary:

During the biotinylation reaction, plastic rings (inner diameter, 1.4 cm) and agarose were used to delimit the regions of biotinylation on the solid surface and to protect the remaining areas from biotinylation, respectively.
We previously showed that biotin moieties can be attached covalently to the outer surface of adenoviral vectors without appreciable effect on their infectivity under carefully controlled biotinylation conditions [ 10 11 ] . Such biotinylated viral particles could be attached, specifically and stably, to a solid surface on which (strept)avidin is covalently immobilized (avidin- or streptavidin-coated solid surface).
The reaction mixtures were incubated on ice in the dark for 2 h, and the biotinylation reaction was terminated by the addition of 100 μl of 9 mM glycine in PBS (pH 7.4).
Detection antibodies (all from Dako; 1:5,000 for 1 h at room temperature) were mouse-anti-goat Ig, mouse-anti-rat Ig, rabbit-anti-mouse Ig, and porcine-anti-rabbit Ig-HRP [ 66 ] . To visualize all transferred proteins, we used the ECL protein biotinylation labeling modules (RPN 2202, Amersham) and streptavidin alkaline phosphatase (V020402, Amersham) in accordance with the manufacturer's instructions.
Biotinylation of Ad5.CMV-LacZ was performed by using sulfo-NHS-LC-biotin (Pierce Chemical, Rockford, IL), a biotin derivative containing an N -hydroxysuccinimidyl ester (NHS) that reacts with primary amino groups.