Example sentences for: biotinylation

How can you use “biotinylation” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Ad5.CMV-LacZ was treated at varying concentrations (0 - 100 μg/ml) of sulfo-NHS-LC-biotin on ice for 2 h in PBS (pH 7.4), followed by the removal of non-virion-associated biotinylation reagent by ultrafiltration.

  • Biotinylation of Ad5.CMV-LacZ was performed by using sulfo-NHS-LC-biotin (Pierce Chemical, Rockford, IL), a biotin derivative containing an N -hydroxysuccinimidyl ester (NHS) that reacts with primary amino groups.

  • These results suggest that, upon biotinylation of the viral surface under controlled conditions, adenoviral vectors can be immobilized on a solid surface by using the (strept)avidin-biotin interaction, and that such immobilized adenoviral vectors are capable of infecting cells, in situ , on the solid surface.

  • Cytoskeletally anchored α V β 6 was immunoprecipitated after TGFβ 1 stimulation (10 nM for 10 minutes) followed by Western analysis with antibodies against tyrosine-phosphorylated proteins (C) or Western blotting after biotinylation of all proteins and streptavidin detection (D).

  • The reaction mixtures were incubated on ice in the dark for 2 h, and the biotinylation reaction was terminated by the addition of 100 μl of 9 mM glycine in PBS (pH 7.4).


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