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Example sentences for: biowhittaker

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These cell lines were grown at 37°C in a humidified atmosphere containing 5% CO 2 . D-17 cells were maintained in Dulbecco's modified Eagles medium (DMEM; BioWhittaker) supplemented with 6% fetal bovine serum (FBS; BioWhittaker), and C6 cells were in DMEM supplemented with 10% FBS.
All amplifications were confirmed by gel electrophoresis (3% Metaphor agarose [Cat # 50181, BioWhittaker Molecular Applications, Rockland, ME] in 0.5X TBE [ 25 ] ). Ethidium bromide-stained products that produced obvious, solitary bands after electrophoresis were processed for size analysis.
The FLS cultures were established from synovial tissues obtained during joint replacement surgery in RA patients [ 6 ] . The FLS were cultured in DMEM plus 10% heat-inactivated human AB serum (BioWhittaker, Walkersville, MD, USA), 10% fetal bovine serum, penicillin, streptomycin, and L-glutamine.
For induction of TGF-β 1 , spleen cells were cultured in serum-free medium X-Vivo-20 (BioWhittaker, Inc., Walkersville, MD, USA) in the presence of proteoglycan.
Cells were cultured in DMEM/F-12 medium (Sigma) supplemented with 20% (v/v) FCS (Biowhittaker Inc., Walkersville, MD) under 5% CO 2 in air at 37°C.