Words similar to biowhittaker

Example sentences for: biowhittaker

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CMV was grown and harvested from MRC-5 fibroblast cultures (Biowhittaker, Walkersville, MD) and concentrated by ultra-centrifugation (28000 g for 60 minutes at 4°C).
Cells were cultured in DMEM/F-12 medium (Sigma) supplemented with 20% (v/v) FCS (Biowhittaker Inc., Walkersville, MD) under 5% CO 2 in air at 37°C.
All sequencing except SNP detection, which is detailed below, was done with the BigDye terminator sequencing kit (PE Biosystems, Foster City, CA) using LongRanger premixed gels (FMC/BioWhittaker) on an Applied Biosystems 377-XL96 DNA sequencer.
The FLS cultures were established from synovial tissues obtained during joint replacement surgery in RA patients [ 6 ] . The FLS were cultured in DMEM plus 10% heat-inactivated human AB serum (BioWhittaker, Walkersville, MD, USA), 10% fetal bovine serum, penicillin, streptomycin, and L-glutamine.
THP-1 (generously provided by Dr P Guyre, Dartmouth Medical School), Jurkat (ATCC), and 293 cell lines were cultured in RPMI complete medium (CM), consisting of RPMI 1640 with 10% heat-inactivated fetal bovine serum, 0.1 ml of sodium pyruvate, 2.0 mmol/l of L-glutamine, 25 mmol/l of 1 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid), 0.1 mmol/l of nonessential amino acids, 0.25 μg/ml of amphotericin, 50 μg/ml gentamicin (all from BioWhittaker, Walkersville, MD, USA) and 5 × 10 -5M 2-mercaptoethanol (Sigma, St Louis, MO, USA).