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Example sentences for: biowhittaker

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These cell lines were grown at 37°C in a humidified atmosphere containing 5% CO 2 . D-17 cells were maintained in Dulbecco's modified Eagles medium (DMEM; BioWhittaker) supplemented with 6% fetal bovine serum (FBS; BioWhittaker), and C6 cells were in DMEM supplemented with 10% FBS.
Dexamethasone, ascorbic acid-2 phosphate, β-glycerophosphate, bovine serum albumin (BSA), and FCS were purchased from Sigma Diagnostics (St Louis, MO, USA); penicillin, streptomycin, DMEM, and RPMI-1640 from Biowhittaker (Watersville, MD, USA); Lymphoprep ™from Nycomed, Oslo, Norway.
All amplifications were confirmed by gel electrophoresis (3% Metaphor agarose [Cat # 50181, BioWhittaker Molecular Applications, Rockland, ME] in 0.5X TBE [ 25 ] ). Ethidium bromide-stained products that produced obvious, solitary bands after electrophoresis were processed for size analysis.
Cell culture medium, nutrients and antibiotics were purchased from BioWhittaker (Walkersville, MD).
For induction of TGF-β 1 , spleen cells were cultured in serum-free medium X-Vivo-20 (BioWhittaker, Inc., Walkersville, MD, USA) in the presence of proteoglycan.