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Example sentences for: biovendor
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Direct comparison of standard curves generated by the Linco kit with those yielded by another commercially available resistin ELISA (Biovendor Laboratory Medicine, Brno, Czech Republic) yielded high correlation (rho = 0.99, p <0.
001), except that the Biovendor values were approximately 30% lower than those determined with the Linco assay.
The HCMV DNA was detected using HCMV1 (5' cct agt gtg gat gac cta cgg gcc a) and HCMV2 (5' cag aca cag tgt cct ccc gct cct c) primers producing 249 bp long amplicon and the DNA of HHV6 was amplified with specific primer pair HP0 (5' ccg caa tcg aat cca cct agc gg) and HP4 (5' gtg aga acg gat tcg aac agt gct g) yielding 440 bp product [ 23 24 ] . All amplifications were carried out with 20 pmol of each primer in 2 mM solution of MgCl 2 , 2 U of Tag Special DNA polymerase (Biovendor, Czech Republic), 0.3 mM of each dNTPs, 10× reaction buffer and 1 μg of isolated DNA according to the following conditions: 96°C for 4 min, (94°C for 10 sec, 58°C for 10 sec, 72°C for 20 sec) 36 times, and final extension at 72°C for 2 min.
Discrepant absolute values among different assays, including the Biovendor assay, were recently described by others [22].
Resistin levels in 40 plasma samples were measured using both Linco and Biovendor ELISA kits, with moderate correlation (rho = 0.66).