Words similar to triton
Example sentences for: triton
How can you use “triton” in a sentence? Here are some example sentences to help you improve your vocabulary:
Prior to hybridization, fragmented cRNA in a 6×SSPE-T hybridization buffer (1 M/L NaCl, 10 mM Tris pH 7.6, 0.005% Triton), was heated to 95°C for 5 min, cooled to 40°C, and then loaded onto the Affymetrix probe array cartridge.
4 with 0.1% Triton X-100, insoluble material removed by centrifugation for 10 minutes at 14,000 rpm and soluble GST fusion proteins purified with GST-Sepharose beads (Pharmacia).
This treatment was shown to remove non-NE bound proteins from both the cytoplasm and the nucleus [ 31 ] . Indeed after Triton extraction, GFP-COP1 protein was completely eliminated from the cytoplasm and the nucleoplasm (Fig 3C, +Triton).
Following an overnight incubation in 0.1% Triton X-100 (Tx100, Sigma, St Louis, MO) containing 3% normal goat serum in PB at 4°C, sections were incubated in rabbit polyclonal anti-GnRH antibody (SW-1, 1:5000 in 0.1% Tx100 in PB, generously provided by Dr. S. Wray, NIH) for at least 4 days at 4°C.
Unless stated otherwise, all incubations were at 37°C in a humidity chamber, followed by two PBS plus Triton X-100 (1:500) washes, 2 minutes each.