Example sentences for: triton

How can you use “triton” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Exponentially growing cells on slides were fixed in 4% paraformaldehyde in PEM buffer (80 mM Pipes [pH 7.0], 5 mM EGTA and 2 mM MgCl 2 ) for 30 min, permeabilized in 0.5% Triton X-100 in PEM buffer at room temperature for 15 min and rinsed three times with TBS + 0.1% Tween 20, followed by incubation in 0.5% nonfat dry milk in TBS + 0.1% Tween 20 for 2 h at room temperature.

  • There was no benefit to sensitivity of background by inclusion of Triton, which tended to result in loss of tissue from slides.

  • Cells were washed twice with PBS and lysed with RIPA buffer (1% Triton X-100, 1% NaDOC, 0.1% SDS, 158 mM NaCl, 10 mM Tris pH 7.2, 5 mM NaEDTA and 1% protease inhibitors, added just before use) (Sigma, St. Louis, MO).

  • NIH3T3 cells suspended in PBS were treated with 1 μM staurosporine, 1 μM calyculin A or 100 μM pervanadate and fractionated with 0.5% Triton X-100 or DOTMAC.

  • PBB was freshly prepared in 24 ml aliquots composed of 20 ml A-150 (20 mM Hepes, 150 mM KCl, 10 mM MgCl2 and 1% glycerol), and 2 ml each of phosphate buffered saline supplemented, respectively, with 1% Triton X-100 and 1% NP-40.


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