Words similar to triton
Example sentences for: triton
How can you use “triton” in a sentence? Here are some example sentences to help you improve your vocabulary:
Exponentially growing cells on slides were fixed in 4% paraformaldehyde in PEM buffer (80 mM Pipes [pH 7.0], 5 mM EGTA and 2 mM MgCl 2 ) for 30 min, permeabilized in 0.5% Triton X-100 in PEM buffer at room temperature for 15 min and rinsed three times with TBS + 0.1% Tween 20, followed by incubation in 0.5% nonfat dry milk in TBS + 0.1% Tween 20 for 2 h at room temperature.
There was no benefit to sensitivity of background by inclusion of Triton, which tended to result in loss of tissue from slides.
Cells were washed twice with PBS and lysed with RIPA buffer (1% Triton X-100, 1% NaDOC, 0.1% SDS, 158 mM NaCl, 10 mM Tris pH 7.2, 5 mM NaEDTA and 1% protease inhibitors, added just before use) (Sigma, St. Louis, MO).
NIH3T3 cells suspended in PBS were treated with 1 μM staurosporine, 1 μM calyculin A or 100 μM pervanadate and fractionated with 0.5% Triton X-100 or DOTMAC.
PBB was freshly prepared in 24 ml aliquots composed of 20 ml A-150 (20 mM Hepes, 150 mM KCl, 10 mM MgCl2 and 1% glycerol), and 2 ml each of phosphate buffered saline supplemented, respectively, with 1% Triton X-100 and 1% NP-40.