Words similar to trituration

Example sentences for: trituration

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Primary neuronal cultures of feotal rat forebrains were prepared as described previously [ 14 ] . Briefly, the forebrains of feotal rat pups (embryonic day 15) were isolated, and the cells were dispersed by repeated mechanical trituration in neuronal culture medium (NCM = Ham's F-12: Basal Medium Eagle, 1:1; supplemented with dextrose, 0.6 g/l; glutamine, 0.35%; and Pen-Strep, 1%).
RPE cells were then removed under a dissecting microscope, dissociated by trituration using a Pasteur pipette, and then plated on poly-L-lysine (25 mg/liter in water) coated 35-cm 2culture dishes.
Cell lysates were obtained by rinsing 12-day old neuronal cell cultures with ice-cold phosphate-buffered saline prior to trituration in ice-cold lysis buffer (PBS supplemented with 1% Igepal, 0.5% sodium deoxycholate, 0.1% SDS, 100 μg/ml PMSF and 300 μg/ml aprotinin).
The tissue was then dissociated by gentle trituration using flamed Pasteur pipets (Fisher; Cat.
Oocytes were prepared after the method of Rho et al . [ 29 ] . Typically 30 oocytes were lysed by trituration in 200 μL of a solution containing 100 mM Tris-HCl, 100 mM NaCl, 0.5% Triton X-100, 0.05 mg/mL pepstatin, 0.05 mg/mL leupeptin, and 0.05 mg/mL aprotinin (pH 8.0) and the homogenate was incubated for 15 minutes on ice.