Words similar to tritiated
Example sentences for: tritiated
How can you use “tritiated” in a sentence? Here are some example sentences to help you improve your vocabulary:
Incorporation of tritiated lysine into tRNA was measured by modification of the protocol of Hou [ 51 ] . Reactions contained 10 micrograms of total protein in 20 mM KCl, 10 mM MgCl 2 , 4 mM dithiothreitol, 2 mM ATP, 50 mM Na HEPES pH 7.5, 20 μM lysine (10 μCi 3H lysine), and were performed with or without 15 μg yeast tRNA (Sigma) in a total volume of 60 μl.
To perform this experiment we synthesized a tritiated diazirine derivative of Hh-Ag 1.2 with an EC 50 in the cell-based assay of 35 nM (data not shown).
Tritiated estradiol glucuronide was added as an internal standard and 700 μl of methanol were mixed with samples.
To explore this possibility we tested whether a tritiated form of the agonist analog Hh-Ag 1.5 could form a complex with Smo, when Smo is transiently overexpressed in 293T cells.
On day 5, cells were pulsed with tritiated thymidine at a concentration of 0.5 μCi/well.