Example sentences for: tritiated

How can you use “tritiated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Incorporation of tritiated lysine into tRNA was measured by modification of the protocol of Hou [ 51 ] . Reactions contained 10 micrograms of total protein in 20 mM KCl, 10 mM MgCl 2 , 4 mM dithiothreitol, 2 mM ATP, 50 mM Na HEPES pH 7.5, 20 μM lysine (10 μCi 3H lysine), and were performed with or without 15 μg yeast tRNA (Sigma) in a total volume of 60 μl.

  • To perform this experiment we synthesized a tritiated diazirine derivative of Hh-Ag 1.2 with an EC 50 in the cell-based assay of 35 nM (data not shown).

  • Tritiated estradiol glucuronide was added as an internal standard and 700 μl of methanol were mixed with samples.

  • To explore this possibility we tested whether a tritiated form of the agonist analog Hh-Ag 1.5 could form a complex with Smo, when Smo is transiently overexpressed in 293T cells.

  • On day 5, cells were pulsed with tritiated thymidine at a concentration of 0.5 μCi/well.


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