Words similar to tritonx-
Example sentences for: tritonx-
How can you use “tritonx-” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cells were fixed for 15 min in 3% (w/v) paraformaldehyde, rinsed for 5 min with 0.05 M NH 4 Cl 2 , permeabilized for 2 min with 0.1% (v/v) TritonX-100, and blocked for 30 min with 0.1% (w/v) BSA.
Following color development, the embryos were treated with 2 N NaOH for 1 hr, washed with PBSTx (phosphate buffered saline with 0.01% TritonX-100), blocked 1 hr in PBDTx (PBS, 1% DMSO, 0.01% TritonX-100 plus 2% sheep serum and 2 mg/ml BSA, then incubated 2 hr with anti-BrdU (monoclonal G3G4, Developmental Studies Hybridoma Bank).
5, 150 mM NaCl, 0.1% TritonX-100 and 0.1% SDS, by passaging through a 25-gauge needle.
A solution of 0.1% TritonX-100 was then used to permeabilize the cells by treatment for 10 min at 4°C.
Cells were fixed in a permeabilization/fixation buffer (100 mM K-Pipes pH 6.8, 10 mM EGTA, 1 mM MgCL2, 0.2% TritonX-100, 3.7% formaldehyde) for 10 minutes.