Words similar to tetrazolium
Example sentences for: tetrazolium
How can you use “tetrazolium” in a sentence? Here are some example sentences to help you improve your vocabulary:
Alkaline phosphatase-conjugated secondary antibody (goat anti-rabbit immunoglobulin G; Pierce) was used to visualize proteins by development with nitroblue tetrazolium and 5-bromo-4-chloro-3-indolylphosphate (NBT-BCIP; Fisher Scientific, Fair Lawn, NJ).
The HL-60 human promyelocytic leukemia cell line responds in culture to sub-micromolar concentrations of retinoic acid ( all-trans and 13-cis ) by undergoing terminal differentiation to a granulocytic cell with the ability to phagocytose and reduce nitroblue tetrazolium [ 28 ] . The response to retinoic acid in HL-60 cells is dependent on the expression of the retinoic acid receptor RAR α [ 29 ] . The use of all-trans retinoic acid in differentiation therapy for patients with acute promyelocytic leukemia has resulted in prolonged, complete remissions when combined with cytotoxic chemotherapy, and may provide complete, long-term remissions as a single agent [ 30 ] .
Cell differentiation was measured by mixing 100 μL aliquots of cells with 100 μL of a solution containing 1.0% w/v bovine serum albumin, 0.2% w/v nitroblue tetrazolium and 1.0 μg/mL of 12-O-tetradecanoylphorbol 13-acetate (TPA).
NAD, all-trans retinal, all-trans retinoic acid, dimethylsulfoxide, mammalian protease inhibitor cocktail, nitroblue tetrazolium, and pyrazole were obtained from Sigma Chemical Co., St. Louis, MO.
For colorimetric detection, hybridization with the targets was revealed by reacting the tissue sections with anti-DIG-alkaline phosphatase conjugate, followed by reaction with nitroblue tetrazolium chloride (NBT), 5-bromo-4-chloro-3-indolyl phosphate (BCIP), and levamisole as previously described [ 39].
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