Words similar to tetraploid
Example sentences for: tetraploid
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Tetraploid aggregation and β-galactosidase staining
However, pioneering work by Nagy et al has shown that embryos and mice can be derived directly from R1 ES cells by aggregating them with tetraploid embryos [ 7 8 ] . This suggests that controlled expression of transgenes encoding possible gain-of-function and developmental-lethal alleles could be achieved by generating embryos and/or mice from ES cells containing transgenes inserted into the hprt locus.
Nagy et al demonstrated that embryos and mice could be derived solely from R1 ES cells by aggregating them with tetraploid embryos [ 7 8 ] . Although we were able to repeat these data using R1 ES cells we were unable to generate midgestation embryos from E14TG2a-derived strains of hprt negative ES cell lines using the same approach [ 9 10 11 ] . We believed it was unlikely that Hprt itself was required to generate ES cell-derived embryos because hprt knockout mice are viable and fertile [ 12 ] . E14TG2a-derived ES cell lines contain a large spontaneous deletion encompassing 55 kb of the hprt locus [ 13 ] . Therefore, it seemed possible that sequences within this region were necessary to maintain ES cell pluripotency.
However, if a line of "transgenic" ES cells can be established then it is possible to generate clonal embryos directly from these ES cells by aggregating them with tetraploid embryos.
In sum, the availability of tetraploid aggregation-competent ES cells that support targeting of transgenes to the hprt locus should facilitate the rapid analysis of transcriptional regulatory elements in vivo as well as the phenotypic characterization of alleles that are incompatible with normal development.
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